Alpha fetoprotein antagonises benzyl isothiocyanate inhibition of the malignant behaviors of hepatocellular carcinoma cells.
Alpha fetoprotein antagonises benzyl isothiocyanate inhibition of the malignant behaviors of hepatocellular carcinoma cells.
复制标题
甲胎蛋白拮抗异硫氰酸苄酯对肝癌细胞恶性行为的抑制作用
DOI：
10.18632/oncotarget.12407
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发表时间：
2016-11-15
期刊：
影响因子：
--
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Zhu M;Li W;Guo J;Lu Y;Dong X;Lin B;Chen Y;Zhang X;Li M
异硫氰酸苄酯（BITC）是一种来源于十字花科蔬菜的膳食异硫氰酸酯。最近的研究表明，BITC抑制许多癌细胞的生长，包括肝细胞癌（HCC）细胞。甲胎蛋白（Alpha-fetoprotein，AFP）是促进肝癌进展的重要分子，本研究探讨AFP对BITC在肝癌细胞恶性行为中的作用的影响及其可能机制。体外实验发现BITC对人肝癌细胞系Bel 7402（AFP产生细胞）和HLE（non-AFP产生细胞）的存活、迁移、侵袭能力有抑制作用，并诱导细胞凋亡。BITC的作用机制可能与促进caspase-3和PARP-1的表达，增强caspase-3活性，降低MMP-2/9、Survivin和CXCR 4的表达有关。AFP可拮抗BITC的作用。这项研究表明，BITC诱导肝癌细胞系的活力显着降低。BITC可激活caspase-3信号，抑制生长转移相关蛋白的表达; AFP是BITC耐药的关键分子。
Benzyl isothiocyanate (BITC) is a dietary isothiocyanate derived from cruciferous vegetables. Recent studies showed that BITC inhibited the growth of many cancer cells, including hepatocellular carcinoma (HCC) cells. Alpha-fetoprotein (AFP) is a important molecule for promoting progression of HCC, in the present investigation, we explore the influence of AFP on the role of BITC in the malignant behaviours of HCC cells, and the potential underlying mechanisms. We found thatBITC inhibited viability, migration, invasion and induced apoptosis of human liver cancer cell lines, Bel 7402(AFP producer) and HLE(non-AFP producer) cells in vitro. The role of BITC involve in promoting actived-caspase-3 and PARP-1 expression, and enhancing caspase-3 activity but decreasing MMP-2/9, survivin and CXCR4 expression. AFP antagonized the effect of BITC. This study suggests that BITC induced significant reductions in the viability of HCC cell lines. BITC may activate caspase-3 signal and inhibit the expression of growth- and metastasis-related proteins; AFP is an pivotal molecule for the HCC chemo-resistance of BITC.