Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis
Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis
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DOI：
10.1186/1471-2164-9-364
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发表时间：
2008-07-31
期刊：
影响因子：
4.4
通讯作者：
Munderloh, Ulrike G.
中科院分区：
文献类型：
--
作者：
Nelson, Curtis M.;Herron, Michael J.;Munderloh, Ulrike G.
背景资料：嗜吞噬细胞无形体（Anaplasma phagocytophilum，Ap）是一种专性胞内细菌，是引起人类粒细胞无形体病的病原体。Ap交替感染蜱和哺乳动物以及每种动物体内的各种细胞类型。了解这种多功能细胞寄生背后的生物学可以通过使用平铺微阵列来建立高分辨率，全基因组的生物体的转录谱，因为它感染的细胞系代表其生命周期（蜱; ISE 6）和发病机制（人类; HL-60和HMEC-1）。在蜱（ISE 6）和人（HL-60和HMEC-1）细胞系之间存在广泛的Ap基因差异转录，而在人细胞系之间差异转录的基因要少得多，并且都不成比例地由膜或表面蛋白代表。在每个细胞系中有Ap基因专门转录，明显的人类和蜱特异性操纵子和旁系同源物，以及反义转录物，表明新的表达调控过程。7个virB 2旁系同源物（细菌IV型分泌系统）显示人类或蜱细胞依赖性转录。以前未被识别的基因和编码序列被鉴定，表达的p44/msp 2（主要表面蛋白）旁系同源物（共114例），通过在每个独特的高变区产生升高的信号--HL-60中2/114例，HMEC-1中3/114例，而ISE 6中无一例。使用这些方法，可以在任何宿主细胞和细胞感染过程的所有阶段中产生Ap以及其他专性细胞内生物体的全基因组转录谱。综合转录数据的视觉表示与基因组的注释图一起将复杂的转录呈现为可辨别的模式。
Background: Anaplasma phagocytophilum (Ap) is an obligate intracellular bacterium and the agent of human granulocytic anaplasmosis, an emerging tick-borne disease. Ap alternately infects ticks and mammals and a variety of cell types within each. Understanding the biology behind such versatile cellular parasitism may be derived through the use of tiling microarrays to establish high resolution, genome-wide transcription profiles of the organism as it infects cell lines representative of its life cycle (tick; ISE6) and pathogenesis (human; HL-60 and HMEC-1).Results: Detailed, host cell specific transcriptional behavior was revealed. There was extensive differential Ap gene transcription between the tick (ISE6) and the human (HL-60 and HMEC-1) cell lines, with far fewer differentially transcribed genes between the human cell lines, and all disproportionately represented by membrane or surface proteins. There were Ap genes exclusively transcribed in each cell line, apparent human-and tick-specific operons and paralogs, and anti-sense transcripts that suggest novel expression regulation processes. Seven virB2 paralogs (of the bacterial type IV secretion system) showed human or tick cell dependent transcription. Previously unrecognized genes and coding sequences were identified, as were the expressed p44/msp2 (major surface proteins) paralogs (of 114 total), through elevated signal produced to the unique hypervariable region of each-2/114 in HL-60, 3/114 in HMEC-1, and none in ISE6.Conclusion: Using these methods, whole genome transcription profiles can likely be generated for Ap, as well as other obligate intracellular organisms, in any host cells and for all stages of the cell infection process. Visual representation of comprehensive transcription data alongside an annotated map of the genome renders complex transcription into discernable patterns.