Full-length genome of a novel genotype 3 hepatitis E virus strain obtained from domestic pigs in Japan
Full-length genome of a novel genotype 3 hepatitis E virus strain obtained from domestic pigs in Japan
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DOI：
10.1016/j.virusres.2017.08.003
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发表时间：
2017-08-15
期刊：
影响因子：
5
通讯作者：
Okamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Primadharsini, Putu Prathiwi;Miyake, Masao;Okamoto, Hiroaki
戊型肝炎病毒（HEV）会引起人类急性或慢性肝炎，并可通过粪口途径传播。猪是这种感染的主要宿主之一。日本猪群中的 60 头猪（4-5 个月大）可检测到抗 HEV IgG 抗体，其中 5 头（8.3%）持续感染基因型 3 HEV。从病毒血症猪中获得的 5 株 HEV 毒株具有 98.8-100% 的核苷酸同一性，其中一种代表性毒株 (swHE1606845) 的整个基因组序列在本研究中确定，与已报道的 3a-3k 亚型 HEV 毒株的差异为 14.1-19.6%，与亚型尚未确定的其他基因型 3 HEV 毒株的差异为 14.7-19.1%。分配的。 swHE1606845 对于亚型 3a-3k 的基因型 3 HEV 毒株显示出较高的核苷酸 p 距离值 >= 0.143，对于未指定亚型的其他基因型 3 毒株显示 >= 0.152。 SimPlot 分析显示缺乏重组事件。这些结果表明 swHE1606845 是基因型 3 的新亚型的候选成员。需要进一步努力鉴定 swHE1606845 样新菌株，以澄清该菌株的起源，并确定另外两个 swHE1606845 样菌株的完整核苷酸序列，以指定新亚型。
Hepatitis E virus (HEV) causes acute or chronic hepatitis in humans and can be transmitted via the fecal-oral route. Pigs are one of the main reservoirs for this infection. Sixty pigs, 4-5 months of age, on a swine herd in Japan had detectable anti-HEV IgG antibodies, and five (8.3%) of them had ongoing infection of genotype 3 HEV. Five HEV strains obtained from the viremic pigs shared 98.8-100% nucleotide identity, and one representative strain (swHE1606845), whose entire genomic sequence was determined in this study, differed by 14.1-19.6% from the reported HEV strains of subtypes 3a-3k and by 14.7-19.1% from other genotype 3 HEV strains whose subtypes have not yet been assigned. swHE1606845 showed a higher nucleotide p-distance value of >= 0.143 with the genotype 3 HEV strains of subtypes 3a-3k and >= 0.152 with other genotype 3 strains of unassigned subtypes. A SimPlot analysis revealed a lack of recombination events. These results indicate that swHE1606845 is a candidate member of a novel subtype of genotype 3. Further efforts to identify the swHE1606845-like novel strain are warranted to clarify the origin of this strain and to determine the complete nucleotide sequences of two additional swHE1606845-like strains for assigning a new subtype.