CLONED AND EXPRESSED MACROPHAGE NITRIC-OXIDE SYNTHASE CONTRASTS WITH THE BRAIN ENZYME
CLONED AND EXPRESSED MACROPHAGE NITRIC-OXIDE SYNTHASE CONTRASTS WITH THE BRAIN ENZYME
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DOI：
10.1073/pnas.89.15.6711
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发表时间：
1992-08-01
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
LOWENSTEIN, CJ;GLATT, CS;SNYDER, SH
一氧化氮(NO)是巨噬细胞、血管内皮细胞和神经元的信使分子。一氧化氮合酶(NOS)的一种神经元形式已经被克隆。我们现在报道巨噬细胞一氧化氮合酶的分子克隆。巨噬细胞酶的序列与神经性酶有50%的同源性。与神经元型一氧化氮合酶一样，巨噬细胞一氧化氮合酶也有FAD、FMN和NADPH的识别位点，也有共同的钙调蛋白结合位点。巨噬细胞一氧化氮合酶基因的诱导性很强，在静止期的巨噬细胞或脾中不表达，但在内毒素处理2-6小时后明显表达。
Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.