Identification of Transcriptional and Receptor Networks That Control Root Responses to Ethylene
Identification of Transcriptional and Receptor Networks That Control Root Responses to Ethylene
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DOI：
10.1104/pp.17.00907
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发表时间：
2018-03-01
期刊：
影响因子：
7.4
通讯作者：
Muday, Gloria K.
中科院分区：
文献类型：
--
作者：
Harkey, Alexandria F.;Watkins, Justin M.;Muday, Gloria K.
高时间分辨率的转录分析为荷尔蒙反应网络提供了实质性的新见解。这项研究确定了全基因组转录丰度变化的动力学响应于植物激素乙烯水平的升高，从轻生长的拟南芥(Arabiopsis Thaliana)幼苗的根中，这些激素水平覆盖在与时间匹配的发育变化上。对具有相似时间模式的转录簇的功能注释显示，具有已知乙烯功能的快速诱导簇和具有与根发育相关的新预测功能的较慢调节的簇。与对暗生长幼苗的研究不同，在暗生长幼苗中，典型的乙烯反应转录因子EIN3在乙烯介导的发育中处于中心地位，而EIN3和EIL1单突变和双突变的根在光生长幼苗中仍然对乙烯做出反应。此外，这些乙烯反应转录子的子集富含EIN3和ERF的靶标。这些结果与EIN3无关的光生长根的发育和转录变化是一致的。对单个和多个功能获得和功能丧失受体突变体的研究表明，在五种乙烯受体中，ETR1控制侧根和根毛的起始和伸长以及其他受体的合成。这些结果为研究光生长幼苗对乙烯的转录和发育反应提供了新的见解。
Transcriptomic analyses with high temporal resolution provide substantial new insight into hormonal response networks. This study identified the kinetics of genome-wide transcript abundance changes in response to elevated levels of the plant hormone ethylene in roots from light-grown Arabidopsis (Arabidopsis thaliana) seedlings, which were overlaid on time-matched developmental changes. Functional annotation of clusters of transcripts with similar temporal patterns revealed rapidly induced clusters with known ethylene function and more slowly regulated clusters with novel predicted functions linked to root development. In contrast to studies with dark-grown seedlings, where the canonical ethylene response transcription factor, EIN3, is central to ethylene-mediated development, the roots of ein3 and eil1 single and double mutants still respond to ethylene in light-grown seedlings. Additionally, a subset of these clusters of ethylene-responsive transcripts were enriched in targets of EIN3 and ERFs. These results are consistent with EIN3-independent developmental and transcriptional changes in light-grown roots. Examination of single and multiple gain-of-function and loss-of-function receptor mutants revealed that, of the five ethylene receptors, ETR1 controls lateral root and root hair initiation and elongation and the synthesis of other receptors. These results provide new insight into the transcriptional and developmental responses to ethylene in light-grown seedlings.