Detection of organics using porphyrin embedded nanoporous organosilicas
Detection of organics using porphyrin embedded nanoporous organosilicas
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DOI：
10.1016/j.bios.2006.07.040
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发表时间：
2007-01-15
影响因子：
12.6
通讯作者：
Markowitz, Michael A.
中科院分区：
文献类型：
--
作者：
Johnson-White, Brandy;Zeinali, Mazyar;Markowitz, Michael A.
分子印迹聚合物和二氧化硅已被研究作为受体结合位点模拟物，用于广泛的分离，催化和检测应用，采用转导机制，包括电导，安培和电容。卟啉也是众所周知的传感器组件，由于其分光光度特性的极端敏感性，在其直接环境的变化。我们已经开发了周期性的介孔有机硅（PMO），将卟啉作为目标结合的光学指示剂的材料。该材料结合了分子印迹PMO的稳定性、选择性和高密度结合位点特性与卟啉的灵敏度和选择性。我们证明了结合的对硝基苯酚，对甲酚，2，4，6-三硝基甲苯，和黑索今的卟啉嵌入的PMO与TNT的选择性吸附超过其他分析物。此外，PMO对每种有机物的结合导致掺入卟啉的分光光度特性的独特变化。这些变化可以通过目视检查或通过使用以96孔格式收集的荧光光谱来观察。(c)2006 Elsevier B. V.保留所有权利。
Molecularly imprinted polymers and silica have been studied as receptor binding site mimics for use in a wide range of separation, catalysis, and detection applications employing transduction mechanisms including conductometric, amperometric, and capacitance. Porphyrins are also well known as sensor components due to the extreme sensitivity of their spectrophotometric characteristics to changes in their immediate environment. We have developed periodic mesoporous organosilicas (PMO) which incorporate a porphyrin into the material for use as an optical indicator of target binding. This material combines the stability, selectivity, and high density of binding sites characteristic of the molecularly imprinted PMO with the sensitivity and selectivity of the porphyrin. We demonstrate binding of p-nitrophenol, p-cresol, 2,4,6-trinitrotoluene, and RDX by the porphyrin-embedded PMOs with selective adsorption of TNT over the other analytes. In addition, the binding of each of the organics by the PMO results in unique changes in the spectrophotometric characteristics of the incorporated porphyrin. These changes can be observed by visual inspection or through the use of fluorescence spectra collected in 96-well format. (c) 2006 Elsevier B.V. All rights reserved.