Inhibitory effect of cryptoporic acid E, a product from fungus Cryptoporus volvatus, on colon carcinogenesis induced with N-methyl-N-nitrosourea in rats and with 1,2-dimethylhydrazine in mice.
Inhibitory effect of cryptoporic acid E, a product from fungus Cryptoporus volvatus, on colon carcinogenesis induced with N-methyl-N-nitrosourea in rats and with 1,2-dimethylhydrazine in mice.
复制标题
DOI：
10.1111/j.1349-7006.1992.tb01987.x
复制
发表时间：
1992-08
期刊：
影响因子：
--
通讯作者：
Asakawa Y
中科院分区：
文献类型：
--
作者：
Narisawa T;Fukaura Y;Kotanagi H;Asakawa Y
研究了隐孔菌二聚倍半萜类化合物隐花酸E(CPA-E)对结肠癌的抑制作用。雌性F344大鼠在第1周和第2周每周3次直肠内注射2 mg N-甲基-N-亚硝脲，第3周开始饲喂含0.2%CPA-E的饲料。雌性ICR小鼠在第1~15周每周腹腔注射10 mg/kg体重的1，2-二甲基肼，从第1周开始饲喂含0.06%CPA-E的饲料。大鼠实验于第35周结束，小鼠于第25周结束。与对照组相比，CPA-E组大鼠的肿瘤发生率和每只动物的肿瘤数量减少：分别为31%和75%(P<；0.05)和0.4±0.2(扫描电子显微镜)和0.9±0.2(0.1>；P>；0.05)；在小鼠(每组16只)中分别为31%和63%(0.1>；P>；0.05)和0.4±0.2(P<；0.05)。经直肠内注射脱氧胆酸诱导的结肠粘膜鸟氨酸脱羧酶活性在饲喂CPA-E的动物中显著低于对照组。这表明CPA-E对结肠癌的发生具有抗促进作用。因此，CPA-E对两种不同结肠癌致癌物处理的大鼠和小鼠的结肠癌均有抑制作用。
The antitumorigenic effect of cryptoporic acid E (CPA‐E), a dimeric drimane sesquiterpenoid isolated from the fungus Cryptoporus volvatus, on colon carcinogenesis was investigated. Female F344 rats given an intrarectal instillation of 2 mg of N‐methyl‐N‐nitrosourea 3 times weekly in weeks 1 and 2 were fed diet containing 0.2% CPA‐E from week 3. Femal ICR mice given 15 weekly intraperitoneal injections of 10 mg of 1,2‐dimethylhydrazine/kg body weight during weeks 1 to 15 were fed diet containing 0.06% CPA‐E from week 1. The experiment was terminated at week 35 for rats and at week 25 for mice. The incidence and the number of tumors per animal were reduced in CPA‐E‐fed animals compared to the controls: 31% vs. 75% (P<0.05) and 0.4±0.2 (SEM) vs. 0.9 ± 0.2 (0.1> P>0.05) in rats, and 31% vs. 63% (0.1>P>0.05) and 0.4±0.2 vs. 2.4 ± 0.8 (P<0.05) in mice (16 animals in each group). Intrarectal deoxycholic acid‐induced colonic mucosal ornithine decarboxylase activity was significantly lowered in CPA‐E‐fed animals compared to controls. This shows an antipromoting activity of CPA‐E against colon carcinogenesis. Thus, it was concluded that CPA‐E inhibits colon cancer development in both rats and mice treated with 2 different colon carcinogens.