Comparison of piggyBac transposition efficiency between linear and circular donor vectors in mammalian cells
Comparison of piggyBac transposition efficiency between linear and circular donor vectors in mammalian cells
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DOI：
10.1016/j.jbiotec.2011.05.009
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发表时间：
2011-07-20
影响因子：
4.1
通讯作者：
Hashida, Mitsuru
中科院分区：
文献类型：
--
作者：
Nakanishi, Hideyuki;Higuchi, Yuriko;Hashida, Mitsuru
piggyBac转座子最近作为一种在哺乳动物细胞中进行转基因整合的工具引起了人们的关注。然而，先前涉及piggyBac的研究仅研究了圆形DNA的转位，尽管一些线性DNA载体用于转染哺乳动物细胞。在这项研究中，我们比较了piggyBac在线性和圆形DNA之间的转座效率。菌落计数试验、荧光素酶试验和质粒拯救试验表明，piggyBac转座子可以从线性DNA转座，但转座效率低于从环状DNA转座的效率。这些结果表明，环状DNA比线性DNA更适合作为piggyBac的供体载体。(C) 2011 Elsevier b.v.版权所有
The piggyBac transposon has recently attracted attention as a tool for transgene integration in mammalian cells. However, previous studies involving piggyBac investigated only transposition from circular DNA, although some linear DNA vectors are used to transfect mammalian cells. In this study, we compared the transposition efficiency of piggyBac between linear and circular DNA. Colony counting assay, luciferase assay, and plasmid rescue assay showed that piggyBac transposon can transpose from linear DNA, but its efficiency is lower than the transposition efficiency from circular DNA. These results suggest that circular DNA is more suitable as donor vectors of piggyBac than linear DNA. (C) 2011 Elsevier B. V. All rights reserved.