Differential distribution of four hyperpolarization-activated cation channels in mouse brain
Differential distribution of four hyperpolarization-activated cation channels in mouse brain
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DOI：
10.1515/bc.1999.121
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发表时间：
1999-07-01
影响因子：
3.7
通讯作者：
Ludwig, A
中科院分区：
文献类型：
--
作者：
Moosmang, S;Biel, M;Ludwig, A
在多种神经元中观察到超极化激活的阳离子电流，称为 Ii。编码超极化激活环核苷酸门控阳离子通道 (HCN1-4) 的基因家族的四个成员已被克隆，使用原位杂交研究了四种 HCN 通道类型在小鼠大脑中的区域表达和细胞定位。 HCN1的表达仅限于嗅球、大脑皮层、海马、上丘和小脑。相比之下，HCN2转录本在大脑中几乎无处不在，信号最强，在嗅球、海马、丘脑和脑干中可见，HCN3在整个大脑中均匀表达，水平非常低，最后，HCN4转录本在丘脑和嗅球中选择性显着表达。一些神经元表达两个或多个HCN通道转录本，包括海马锥体神经元（HCN1、HCNP和低水平的HCN 4）和丘脑中继神经元（HCNP和HCN4），我们的结果表明每个HCN通道转录本在大脑中具有独特的分布。此外，他们认为神经元 lh 的异质性可能至少部分归因于 HCN 通道基因的差异表达。
Hyperpolaritation-activated cation currents, termed I,, are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned, The regional expression and cellular localization of the four HCN channel types in mouse brain was investigated using in situ hybridization. The expression of HCN1 was restricted to the olfactory bulb, cerebral cortex, hippocampus, superior colliculus and cerebellum. In contrast, HCN2 transcripts were found at high levels nearly ubiquitiously in the brain, and the strongest signals were seen in the olfactory bulb, hippocampus, thalamus and brain stem, HCN3 was uniformly expressed at very low levels throughout the brain, Finally, HCN4 transcripts were prominently expressed selectively in the thalamus and olfactory bulb. Some neurons expressed two or more HCN channel transcripts including hippocampal pyramidal neurons (HCN1, HCNP and low levels of HCN 4) and thalamic relay neurons (HCNP and HCN4), Our results demonstrate that each HCN channel transcript has a unique distribution in the brain. furthermore, they suggest that the heterogeneity of neuronal lh may be, at least in part, due to the differential expression of HCN channel genes.