FREQUENCY-DISTRIBUTION OF CYTOMEGALOVIRUS ENVELOPE GLYCOPROTEIN GENOTYPES IN BONE-MARROW TRANSPLANT RECIPIENTS
FREQUENCY-DISTRIBUTION OF CYTOMEGALOVIRUS ENVELOPE GLYCOPROTEIN GENOTYPES IN BONE-MARROW TRANSPLANT RECIPIENTS
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DOI：
10.1093/infdis/169.4.769
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发表时间：
1994-04-01
影响因子：
6.4
通讯作者：
TOROKSTORB, B
中科院分区：
文献类型：
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作者：
FRIES, BC;CHOU, SW;TOROKSTORB, B
采用聚合酶链反应（PCR）扩增DNA的限制性分析，巨细胞病毒（CMV）包膜糖蛋白（gB和gH）基因型确定的病毒分离物从128例骨髓移植受者与致命或非致命的CMV。所有的分离株可以分配到四个gB和gH基因型以前确定的DNA测序研究。gB 1型的分离株更常见于gH 2型，而gB 2-4型更常见于gH 1型。通过对gp 55和gp 116结构域可变区DNA的限制性分析检测到与gB重组的小频率。对29例患者不同部位的多个分离株进行分型，除3例例外，单一患者所有分离株的gB基因型保持不变。CMV感染后存活的患者比死亡的患者更常脱落gB 1型病毒（P = 0.003）。患者亚群之间gB型的这种显著差异尚无法解释，但提出了gB基因型可能作为骨髓移植患者CMV株致病性的标志物的可能性。
Using restriction analysis of polymerase chain reaction (PCR)-amplified DNA, the cytomegalovirus (CMV) envelope glycoprotein (gB and gH) genotypes were determined for virus isolates from 128 bone marrow transplant recipients with fatal or nonfatal CMV. All isolates could be assigned to one of four gB and gH genotypes previously identified by DNA sequencing studies. Isolates of gB type 1 were more commonly found to be of gH type 2, whereas gB types 2-4 were more commonly linked to gH type 1. A small frequency of recombination with gB was detected by restriction analysis of DNA from variable regions of the gp55 and gp116 domains. Multiple isolates from various sites of 29 patients were typed and, with three exceptions, the gB genotype remained constant in all isolates from a single patient. Patients who survived CMV infection more commonly shed virus of gB type 1 than those who died (P = .003). This significant difference of gB types among patient subsets is unexplained but raises the possibility that gB genotypes may serve as a marker for pathogenicity of CMV strains in marrow transplant patients.