Metabolic Conversion of Zearalenone to α-Zearalenol by Goat Tissues
Metabolic Conversion of Zearalenone to α-Zearalenol by Goat Tissues
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DOI：
10.1292/jvms.09-0122
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发表时间：
2010-03-01
影响因子：
1.2
通讯作者：
Kumagai, Susumu
中科院分区：
文献类型：
--
作者：
Dong, Mei;Tulayakul, Phitsanu;Kumagai, Susumu
玉米烯酮(玉蜀黍属)。一种由几种镰刀菌产生的雌性真菌毒素，可转化为一种活性代谢物-玉米赤霉烯醇（α - zol）和一种活性较低的代谢物。为了阐明ZEA在反刍动物体内代谢过程中各组织的作用，本研究对成年公、母山羊不同组织对ZEA的体内代谢转化进行了研究，结果表明ZEA主要在肝脏中代谢。α - zol是胞质组分的主要代谢物。而β - zol是微粒体部分的主要代谢物，酶动力学实验的K-m和V-max值证实了ZEA代谢的这一特征，肝脏线粒体后部分主要将ZEA转化为α - zol。这表明山羊活着，可能是作为一个激活器官而不是作为一个灭活器官。在大多数其他组织包括管腔组织中活动将玉蜀黍属转换为alpha-ZOL高于beta-ZOL alpha-ZOL的数量由胃肠道组织是由肝脏组织的1/8-1/3 m /我的蛋白质,但所有胃肠组织生产的贡献alpha-ZOL估计可比10的肝脏由于反刍动物的胃肠道组织的大质量总体结果显示或不仅肝脏组织的重要性,而且其他组织，尤其是胃肠道组织，也会形成一种强效的雌激素代谢物，α - zol
Zearalenone (ZEA). an estrogenic mycotoxin produced by several Fusarium species, is converted into a mole active metabolite, alpha-zearalenol (alpha-ZOL), and a less active metabolite. beta-zearalenol (beta-ZOL), by liver subcellular fractions, hut evidence of this reaction in other tissues is hunted In order to clarify the role of various tissues in ZEA metabolism in ruminant, we investigated the in vino metabolic conversion of ZEA by various tissues of adult male and female goats The results indicate that in the liver. alpha-ZOL was a major metabolite in cytosolic fractions. whereas beta-ZOL was a predommant metabolite in microsome fractions Such a feature of ZEA metabolism was confirmed by the K-m and V-max values from an enzyme kinetics experiment Post-mitochondrial fractions of the liver convened ZEA predominantly to alpha-ZOL. indicating that the goat live, may function as an activation organ rather than as an inactivation organ. for ZEA metabolism in goats In most other tissues including lumen tissue. the activity converting ZEA to alpha-ZOL was higher than that to beta-ZOL The amount of alpha-ZOL formed by gastrointestinal tissues was 1/8-1/3 of that by the liver tissue m terms of the amount per me protein, but the contribution of all gastrointestinal tissues to production alpha-ZOL was estimated to be comparable 10 that of the liver because of the large mass of gastrointestinal tissues in ruminants Overall the results show the importance or not only the liver tissue, but also other tissues, especially gastrointestinal ones, ID the formation of a potent estrogenic metabolite, alpha-ZOL