Diminished monocyte function in microfilaremic patients with lymphatic filariasis and its relationship to altered lymphoproliferative responses.
Diminished monocyte function in microfilaremic patients with lymphatic filariasis and its relationship to altered lymphoproliferative responses.
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淋巴丝虫病微丝虫血症患者的单核细胞功能下降及其与淋巴增殖反应改变的关系。
DOI：
10.1128/iai.73.6.3385-3393.2005
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发表时间：
2005
影响因子：
3.1
通讯作者：
Narayanan,RB
中科院分区：
文献类型：
--
作者：
Sasisekhar,B;Aparna,M;Augustin,DJ;Kaliraj,P;Kar,SK;Nutman,TB;Narayanan,RB
抗原特异性对丝虫抗原的低反应性是在人类淋巴寄生丝虫感染中观察到的一种现象。这种现象归因于多种因素，其中之一是单核细胞功能的改变。为了研究单核细胞在丝虫病感染中所起的作用，我们评估了从正常和丝虫病感染个体中获得的单核细胞对粗丝虫病抗原和纯化重组丝虫病抗原wbsfp -1的反应，并试图将它们与丝虫病感染中观察到的改变的淋巴细胞增殖反应联系起来。与来自地方性正常人或淋巴水肿患者的单核细胞相比，来自微丝病（MF）患者的单核细胞对脂多糖没有反应。事实上，白细胞介素1β (IL-1β)的产生受到严重限制，这一发现并不适用于单核细胞对丝虫抗原的反应。来自MF患者的血清降低了正常单核细胞的粘附性和扩散，这一发现在其他临床组的血清中未见。有趣的是，IL-1β的产生与依从性之间存在显著相关性。此外，自发产生IL-1β的水平与自发分泌IL-10的高水平相关。观察到的影响不是由于细胞表面标志物CD14和HLA-DR的表达降低或改变。这些数据表明，单核细胞功能在MF患者中受到抑制，这一发现可能会改变专利感染期间的淋巴细胞增殖反应。
Antigen-specific hyporesponsiveness to filarial antigens is a phenomenon observed in patent infection with lymph-dwelling filarial parasites of humans. This phenomenon has been attributed to a multitude of factors, one of which is altered monocyte function. To examine the role played by monocytes in filarial infection, we assessed the responses of monocytes obtained from normal and filarial parasite-infected individuals to both crude filarial antigen and purified recombinant filarial antigen WbSXP-1 and attempted to relate these to the altered lymphoproliferative responses seen in filarial infection. Monocytes from microfilaremic (MF) patients demonstrated an inability to respond to lipopolysaccharide compared to monocytes from endemic normal persons or from lymphedema patients. Indeed, interleukin 1β (IL-1β) production was severely limited, a finding that did not extend to monocyte responses to filarial antigens. Serum from MF patients reduced adherence and spreading of normal monocytes, a finding not seen with serum from the other clinical groups. Interestingly, there was a significant correlation between the production of IL-1β and adherence. Moreover, the levels of spontaneous production of IL-1β correlated with high levels of spontaneous secretion of IL-10. The effects observed were not a result of diminished viability or alteration in the expression of the cell surface markers CD14 and HLA-DR. These data suggest that monocyte function is dampened in MF patients, a finding which could alter lymphoproliferative responses during patent infection.