Genetic characterization of five powdery mildew disease resistance loci in Arabidopsis thaliana
Genetic characterization of five powdery mildew disease resistance loci in Arabidopsis thaliana
复制标题
DOI：
10.1046/j.1365-313x.1996.09030341.x
复制
发表时间：
1996-03-01
期刊：
影响因子：
7.2
通讯作者：
Somerville, SC
中科院分区：
文献类型：
--
作者：
Adam, L;Somerville, SC
本文报道了六种拟南芥种质，它们对白粉病病原体白粉病（Erysiphe cichoracearum）的野生分离株表现出抗性。这些种质在接种后7天的抗性特征是真菌生长有限以及接种部位零星出现褪绿或坏死病变。三个种质，Wa-1、Kas-1 和 SI-0，具有高度抗性，而其他种质允许一些真菌生长和分生孢子。乳头形成是一种常见的宿主反应。然而，细胞死亡似乎既不是对感染的快速反应，也不是常见的反应。为了确定抗性的遗传基础，对每个抗性种质与对白粉病病原体敏感的哥伦比亚（gl1）之间的杂交后代进行了分离分析。对于除 SI-0 之外的所有种质，抗性均由单个基因座赋予。 SI-0 的独特之处在于两个不连锁的基因座控制疾病反应表型。在种质 Wa-1、Kas-1、Stw-0 和 Su-0 中，白粉病抗性由半显性等位基因编码。然而，在Te-0和SI-0种质中，易感性对抗性占主导地位。绘图研究表明，Kas-1、Wa-1、Te-0、Su-0 和 Stw-0 的白粉病抗性由五个独立位点控制。这项研究表明拟南芥白粉病将成为研究白粉病的合适模型系统。
This paper reports on six Arabidopsis accessions that show resistance to a wild isolate of the powdery mildew pathogen, Erysiphe cichoracearum. Resistance at 7 days post-inoculation in these accessions was characterized by limited fungal growth and sporadic development of chlorotic or necrotic lesions at inoculation sites. Three accessions, Wa-l, Kas-1 and SI-0, were highly resistant, while the other accessions permitted some fungal growth and conidiation. Papilla formation was a frequent host response; however, cell death appeared to be neither a rapid nor a common response to infection. To determine the genetic basis of resistance, segregation analyses of progeny from crosses between each of the resistant accessions and Columbia (gl1), which is susceptible to the powdery mildew pathogen, were performed. For all accessions except SI-0, resistance was conferred by a single locus. SI-0 was unique in that two unlinked loci controlled the disease reaction phenotype. In accessions Wa-l, Kas-1, Stw-0 and Su-0, powdery mildew resistance was encoded by a semi-dominant allele. However, susceptibility was dominant to resistance in accessions Te-0 and SI-0. Mapping studies revealed that powdery mildew resistances in Kas-1, Wa-l, Te-0, Su-0 and Stw-0 were controlled by five independent loci. This study suggests that the Arabidopsis powdery mildew disease will be a suitable model system in which to investigate powdery mildew diseases.