Real-time DNA microarrays: reality check.
Real-time DNA microarrays: reality check.
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DOI：
10.1042/bst0370471
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发表时间：
2009-04
影响因子：
3.9
通讯作者：
Blair S
中科院分区：
文献类型：
--
作者：
Chagovetz A;Blair S
DNA微阵列受到不一致的定量和假阳性结果的困扰。利用已建立的表面反应机制，我们认为这些问题是当前技术所固有的。特别是，多重非平衡反应的问题不能在现有范式的框架内得到解决。我们讨论的优点和局限性改变的范例，以实时数据采集类似于实时PCR方法。我们的分析表明，多重反应的基本问题并不能通过实时方法本身来解决。然而，通过引入新的检测化学和分析方法，可以从实时微阵列数据中提取特定目标的定量信息。讨论了实时微阵列的可能应用范围。
DNA microarrays are plagued with inconsistent quantifications and false-positive results. Using established mechanisms of surface reactions, we argue that these problems are inherent to the current technology. In particular, the problem of multiplex non-equilibrium reactions cannot be resolved within the framework of the existing paradigm. We discuss the advantages and limitations of changing the paradigm to real-time data acquisition similar to real-time PCR methodology. Our analysis suggests that the fundamental problem of multiplex reactions is not resolved by the real-time approach itself. However, by introducing new detection chemistries and analysis approaches, it is possible to extract target-specific quantitative information from real-time microarray data. The possible scope of applications for real-time microarrays is discussed.