Translationally controlled tumor protein (TCTP) downregulates Oct4 expression in mouse pluripotent cells
Translationally controlled tumor protein (TCTP) downregulates Oct4 expression in mouse pluripotent cells
复制标题
翻译控制肿瘤蛋白 (TCTP) 下调小鼠多能细胞中的 Oct4 表达
DOI：
10.5483/bmbrep.2012.45.1.20
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发表时间：
2012-01-31
期刊：
影响因子：
3.8
通讯作者：
Wang, Huayan
中科院分区：
文献类型：
--
作者：
Cheng, Xiang;Li, Junhua;Wang, Huayan
本研究旨在探讨翻译控制肿瘤蛋白（TCTP）在小鼠胚胎癌P19细胞和小鼠J1胚胎干（ES）细胞中调控Oct4的功能。体细胞内源TCTP mRNA水平比多能性P19和J1 ES细胞高24倍。小鼠多能性细胞中过表达TCTP不仅降低了Oct4转录水平，还降低了干细胞的多能性。TCTP的n端（氨基酸1-60）在抑制Oct4启动子中起重要作用。此外，P19细胞中TCTP的过表达以剂量和时间依赖的方式抑制Oct4启动子活性。此外，小干扰RNA敲低TCTP可增加Oct4的表达。我们的研究表明，TCTP通过结合Oct4启动子的Sf1位点下调小鼠多能细胞中Oct4的表达。[BMB报道2012；45 (1): 20 - 25)
The present study aimed to investigate the function of translationally controlled tumor protein (TCTP) in the regulation of Oct4 in mouse embryonic carcinoma P19 cells and mouse J1 embryonic stem (ES) cells. The mRNA level of endogenous TCTP in somatic cells was 24 folds higher than that in pluripotent P19 and J1 ES cells. Overexpression of TCTP in mouse pluripotent cells not only reduced the level of Oct4 transcription, but also decreased the pluripotency of stem cells. The N-terminal end of TCTP (amino acids 1-60) played an important role in suppressing the Oct4 promoter. Moreover, overexpression of TCTP in P19 cells suppressed the Oct4 promoter activity in a dose- and a time-dependent manner. In addition, knockdown of TCTP by small interfering RNA increased the expression of Oct4. Our study indicates that TCTP downregulates the Oct4 expression by binding the Sf1 site of Oct4 promoter in mouse pluripotent cells. [BMB reports 2012; 45(1): 20-25]