Opposite-polarity motors activate one another to trigger cargo transport in live cells.-猫眼课题宝

DOI：

10.1083/jcb.200908075

发表时间：

2009-12-28

期刊：

The Journal of cell biology

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Ally S;Larson AG;Barlan K;Rice SE;Gelfand VI

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806283/?report=classic

任何两个相反极性的电机之间的机械相互作用是必要的，足够的双向细胞器运输活细胞。细胞内运输通常是双向的，由一系列来回运动组成。驱动蛋白-1和细胞质动力蛋白需要彼此来沿着微管双向运输细胞内货物;即，驱动蛋白-1的抑制或耗尽消除了动力蛋白驱动的货物运输，反之亦然。使用果蝇S2细胞，我们表明，更换内源性驱动蛋白-1或动力蛋白与一个无关的，过氧化物酶体靶向电机相同的方向激活过氧化物酶体运输在相反的方向。然而，运动缺陷型的马达，保留了结合微管和水解三磷酸腺苷的能力，不激活过氧化物酶体运动。因此，任何一对极性相反的马达，只要它们沿着微管运动，就可以相互激活。这些结果表明，相反极性的电机之间的机械相互作用是必要的和足够的双向细胞器运输活细胞。

Mechanical interactions between any two opposite-polarity motors are necessary and sufficient for bidirectional organelle transport in live cells. Intracellular transport is typically bidirectional, consisting of a series of back and forth movements. Kinesin-1 and cytoplasmic dynein require each other for bidirectional transport of intracellular cargo along microtubules; i.e., inhibition or depletion of kinesin-1 abolishes dynein-driven cargo transport and vice versa. Using Drosophila melanogaster S2 cells, we demonstrate that replacement of endogenous kinesin-1 or dynein with an unrelated, peroxisome-targeted motor of the same directionality activates peroxisome transport in the opposite direction. However, motility-deficient versions of motors, which retain the ability to bind microtubules and hydrolyze adenosine triphosphate, do not activate peroxisome motility. Thus, any pair of opposite-polarity motors, provided they move along microtubules, can activate one another. These results demonstrate that mechanical interactions between opposite-polarity motors are necessary and sufficient for bidirectional organelle transport in live cells.