Effect of Borneol on the Distribution of Danshensu to the Eye in Rabbit via Oral Administration
Effect of Borneol on the Distribution of Danshensu to the Eye in Rabbit via Oral Administration
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DOI：
10.3109/02713681003718091
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发表时间：
2010-07-01
影响因子：
2
通讯作者：
Cui, Hao
中科院分区：
文献类型：
--
作者：
Li, Zhijian;Sun, Dianjun;Cui, Hao
目的：冰片和丹参素均为中药中提取的生物活性物质。本文研究了冰片口服对丹参素在家兔眼内分布的影响。方法：给药15 min后，兔耳外静脉注射丹参素（1.0 g/kg），采用高效液相色谱法测定血浆、房水和玻璃体中丹参素的浓度。采用Draize试验检查眼部刺激。结果：与单用丹参素比较，丹参素与冰片共给药血浆浓度在60.99 ~ 722.90 μ g/ml之间。两组的峰值时间（T-max）均出现在0 min；但与对照组比较，差异无统计学意义（P < 0.05）。实验组丹参素在房水和玻璃体中的浓度均高于对照组。差异有统计学意义（P < 0.05）。两组T-max均在25 min出现。检查时未见刺激或炎症反应。结论：在体内，冰片对药物通过血眼屏障的通透性有明显的促进作用，提示冰片具有作为眼穿透增强剂的潜力。
Purpose: Both borneol and danshensu are bioactive substances derived from traditional Chinese medicine. In this paper, the effect of borneol on the distribution of danshensu to the eyes in rabbits via oral administration was investigated.Methods: The rabbits were injected danshensu (1.0 g/kg) into the auris dextra vein 15 min after intragastric administration of borneol and the danshensu concentrations in plasma, aqueous humor, and vitreous humor were measured by high-performance liquid chromatography. Draize test was used to examine the eye irritation.Results: Compared with the administration of danshensu alone, danshensu concentrations co-administrated with borneol in plasma were between 60.99 and 722.90 mu g/ml. Peak times (T-max) in both groups appeared at 0 min; however, compared with the control group, the values were not statistically significant although they differ (P > 0.05). The concentrations of danshensu in aqueous humor and vitreous humor in test group are higher than that in control group. The difference was statistically significant (P < 0.05). T-max in both groups appeared at 25 min. Neither irritation nor inflammatory reaction was found during the examinations.Conclusion: The results suggest that the promoting effect of borneol on the permeability of drugs through the blood-ocular barrier in vivo is obvious, which indicates that borneol has the potential to be used as an ocular penetration enhancer.