Effects of ethanol on 5-hydroxytryptamine release from rat corpus striatum in vivo.
Effects of ethanol on 5-hydroxytryptamine release from rat corpus striatum in vivo.
复制标题
乙醇对体内大鼠纹状体释放 5-羟色胺的影响。
DOI：
10.1016/0741-8329(85)90054-0
复制
发表时间：
1985
期刊：
影响因子：
2.3
通讯作者：
B. Snape
中科院分区：
文献类型：
--
作者：
R. Holman;B. Snape
用在体透析插管观察了乙醇对大鼠纹状体内5-羟色胺（5-HT）释放的影响。从麻醉大鼠中连续采集15分钟透析液样本长达5.25小时，并通过HPLC-电化学检测分析其5-羟基吲哚乙酸（5-HIAA）含量。乙醇腹腔注射引起5-HT释放的增加，反映为灌注样品中5-HIAA浓度的增加。比较两种剂量的乙醇导致最大血液乙醇浓度为50 mM与80 mM，表明较低剂量引起释放更迅速、持续时间更长且显著增加。结果强调了监测个体动物中与酒精治疗相关的神经化学事件的时间进程的重要性，并证明了体内透析插管技术在该背景下的价值。
The effects of ethanol administration on the release of 5-hydroxytryptamine (5-HT) within the corpus striatum of the rat was investigated usingin vivodialysis cannulae. Fifteen-minute dialysate samples were continuously collected for up to 5.25 hr from individual, urethane-anaesthetized rats and analyzed for their content of 5-hydroxyindoleacetic acid (5-HIAA) by HPLC-electrochemical detection. Ethanol injected intraperitoneally caused an increase in 5-HT release reflected as an increase in the concentrations of 5-HIAA in perfusion samples. Comparison of two doses of ethanol resulting in maximum blood ethanol concentrations of 50 mM versus 80 mM, showed that the lower dose elicited a more rapid, longer lasting and significantly greater increase in release. The results emphasise the importance of monitoring the time courses of neurochemical events associated with alcohol treatment in individual animals, and demonstrate the value of thein vivodialysis cannula technique in that context.