Leptomeningeal-derived doublecortin-expressing cells in poststroke brain.
Leptomeningeal-derived doublecortin-expressing cells in poststroke brain.
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DOI：
10.1089/scd.2011.0657
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发表时间：
2012-09
影响因子：
4
通讯作者：
T. Nakagomi;Z. Molnár;A. Taguchi;Akiko Nakano‐Doi;Shan Lu;Yukiko Kasahara;N. Nakagomi;T. Matsuyama
中科院分区：
文献类型：
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作者：
T. Nakagomi;Z. Molnár;A. Taguchi;Akiko Nakano‐Doi;Shan Lu;Yukiko Kasahara;N. Nakagomi;T. Matsuyama
越来越多的证据表明，神经干/祖细胞（neural stem/progenitor cells，NSPCs）存在于中枢神经系统（central nervous system，CNS）的许多区域，包括侧脑室的室管膜下区（subventricular zone，SVZ）、海马齿状回的颗粒下区、皮质、纹状体和脊髓。使用小鼠模型的皮质梗死，我们最近证明，软脑膜（软脑膜），其中覆盖整个皮质，也表现出NSPC活动，响应缺血。软脑膜缺血诱导的NSPCs表达NSPC标记物，如巢蛋白，形成具有自我更新活性的神经球样细胞簇，并分化为神经元，星形胶质细胞和少突胶质细胞，尽管它们与先前报道的NSPCs，如SVZ星形胶质细胞，室管膜细胞，少突胶质细胞前体细胞和反应性星形胶质细胞不相同。在这项研究中，我们发现中风后大脑中的软脑膜细胞表达未成熟神经元标记物doublecortin以及nestin。我们还发现这些细胞可以迁移到中风后的皮层。因此，软脑膜可能参与中枢神经系统修复脑损伤的反应。
Increasing evidence indicates that neural stem/progenitor cells (NSPCs) reside in many regions of the central nervous system (CNS), including the subventricular zone (SVZ) of the lateral ventricle, subgranular zone of the hippocampal dentate gyrus, cortex, striatum, and spinal cord. Using a murine model of cortical infarction, we recently demonstrated that the leptomeninges (pia mater), which cover the entire cortex, also exhibit NSPC activity in response to ischemia. Pial-ischemia-induced NSPCs expressed NSPC markers such as nestin, formed neurosphere-like cell clusters with self-renewal activity, and differentiated into neurons, astrocytes, and oligodendrocytes, although they were not identical to previously reported NSPCs, such as SVZ astrocytes, ependymal cells, oligodendrocyte precursor cells, and reactive astrocytes. In this study, we showed that leptomeningeal cells in the poststroke brain express the immature neuronal marker doublecortin as well as nestin. We also showed that these cells can migrate into the poststroke cortex. Thus, the leptomeninges may participate in CNS repair in response to brain injury.