Loss of cyclin D1 and p16 expression correlates with local recurrence in nasopharyngeal carcinoma following radiotherapy
Loss of cyclin D1 and p16 expression correlates with local recurrence in nasopharyngeal carcinoma following radiotherapy
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DOI：
10.1093/annonc/mdf215
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发表时间：
2002-08-01
影响因子：
50.5
通讯作者：
Chang, HW
中科院分区：
文献类型：
--
作者：
Hwang, CF;Cho, CL;Chang, HW
背景：cyclin D1/p16/Rb 通路在肿瘤发生中起着至关重要的作用，该通路的每个组成部分都可能在各种恶性肿瘤中受到影响。本研究的目的是探讨这些蛋白在鼻咽癌（NPC）中的表达和预后意义。患者和方法：对 65 名接受鼻咽癌放疗的患者进行了分析。通过对存档的预处理肿瘤材料进行免疫组织化学分析来评估cyclin D1、p16和pRb的表达，并将这些蛋白的表达与临床病理参数相关。结果：在65个NPC中的43个（66%）中观察到cyclin D1的阳性表达。分别在 65 个肿瘤中的 42 个（65%）和 65 个肿瘤中的 4 个（6%）中发现 p16 和 pRb 失活。除 7 个肿瘤外，所有肿瘤（65 个肿瘤中的 58 个，89%）均在细胞周期蛋白 D1/p16/Rb 通路中至少含有一种交替。 cyclin D 1 和 p16 的缺失与鼻咽癌放疗后局部复发密切相关（P=0.015 和 0.047）。 pRb 表达与临床病理结果之间没有明显关联。结论：该研究结果表明细胞周期蛋白 D1/p16/Rb 通路在 NPC 肿瘤发生中发挥重要作用。我们还发现 NPC 中的细胞周期蛋白 D1 和 p16 蛋白水平可能在临床上用作局部肿瘤控制的预测因子。
Background: The cyclin D1/p16/Rb pathway plays a critical role in tumorigenesis and each component of this pathway may be affected in various malignancies. The purpose of this study was to investigate the expression and prognostic significance of these proteins in nasopharyngeal carcinoma (NPC).Patients and methods: Sixty-five patients undergoing radiotherapy for NPC were analyzed. The expression of cyclin D1, p16 and pRb was evaluated with immunohistochemical analysis of archived pretreatment tumor materials and expression of these proteins was correlated with clinicopathological parameters.Results: Positive expression of cyclin D1 was observed in 43 of 65 NPCs (66%). p16 and pRb inactivation was identified in 42 of 65 (65%) and four of 65 (6%) tumors, respectively. All but seven tumors (58 of 65, 89%) contained at least one alternation in the cyclin D1/p16/Rb pathway. Loss of cyclin D 1 as well as p16 was closely related to local recurrence after radiotherapy for NPC (P=0.015 and 0.047). No association between pRb expression and clinicopathological outcome was apparent.Conclusions: The study's results suggest that the cyclin D1/p16/Rb pathway plays an important role: in NPC tumorigenesis. We also find that cyclin D1 and p16 protein levels in NPC may be of use clinically as a predictor of local tumor control.