High frequency of vegetative incompatibility combined with haploid selfing in the native European ash foliage coloniser Hymenoscyphus albidus
High frequency of vegetative incompatibility combined with haploid selfing in the native European ash foliage coloniser Hymenoscyphus albidus
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DOI：
10.1016/j.funeco.2017.04.002
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发表时间：
2017-08-01
期刊：
影响因子：
2.9
通讯作者：
Webber, Joan
中科院分区：
文献类型：
--
作者：
Brasier, Clive;King, Kevin;Webber, Joan
欧洲本土的灰真菌Hymenoscyphus albidus受到形态相似的引入灰枯梢病原体Hymenoscyphus fraxineus的威胁。H.秦皮属是异宗配合的，其种群包括许多不同的营养体相容性（VC）类型。H. albidus为同宗配合，种群多样性未知。体外配对H.来自一个法国和三个英国地点的albidus分离株显示出与H.白蜡树。本地vc多样性高，每个分离物的vc类型从约。0.7到0.9，并且任何两个分离物不相容的概率约为0.9%。93-98%;表明每个群体中至少有四个双等位基因维克基因座。然而，来自同一子囊盘的单子囊孢子分离物之间的配对是一致相容的。所有的轴和单子囊孢子分离物都有MAT 1 -1-3和MAT 1 -2-1交配型基因，与同宗配合一致。结果表明，当地H. albidus种群包括许多部分通过单倍体自交保持的无性系不相容的基因。皇冠版权所有（C）2017由爱思唯尔有限公司发布。保留所有权利。
The native European ash fungus Hymenoscyphus albidus is threatened by the morphologically similar introduced ash dieback pathogen Hymenoscyphus fraxineus. H. fraxineus is heterothallic and its populations comprise numerous different vegetative compatibility (vc) types. H. albidus is homothallic with unknown population diversity. In vitro pairings of H. albidus isolates from one French and three UK sites showed compatible (intermingling) and incompatible (gap) reactions similar to those in H. fraxineus. Local vc diversity was high with vc types per isolate ranging from ca. 0.7 to 0.9 and the probability of any two isolates being incompatible ca. 93-98%; suggesting at least four biallelic vic loci in each population. Pairings between single ascospore isolates from the same apothecium, however, were uniformly compatible. All rachis and single ascospore isolates had both MAT1-1-3 and MAT1-2-1 mating type genes, consistent with homothallism. The results suggest local H. albidus populations comprise numerous, vegetatively incompatible genets maintained in part by haploid selfing. Crown Copyright (C) 2017 Published by Elsevier Ltd. All rights reserved.