Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe
Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe
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通过电子显微镜表征减数分裂缺陷突变体并绘制裂殖酵母裂殖酵母中四个必需基因的图谱
DOI：
10.1007/bf00425432
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Kenji Tanaka
中科院分区：
文献类型：
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作者：
C. Shimoda;A. Hirata;M. Kishida;T. Hashida;Kenji Tanaka
摘要通过电子显微镜和细胞核4′，6-二脒基-2-苯基吲哚染色对携带mei 1，mei 2，mei 3，mei 4和mes 1突变等位基因的裂殖酵母裂殖酵母减数分裂缺陷突变体进行了表征。无论是mei 1，mei 2或mei 3突变体的合子含有一个圆形的核与一个单一的纺锤体极体（SPB）。这些突变体在减数分裂前DNA合成之前被捕获。mei 4突变体的合子有一个细长的核，含有厚的电子致密丝（线性元件）。在mes 1突变体中，第一次减数分裂完成，但SBPs没有复制。通过单倍体化、随机孢子和四分体分析，定位了四个减数分裂必需基因（mei 2、mei 3、mei 4和mes 1）。mei 2基因位于第一染色体上，距离ura 2基因14.2cm。mei 3基因与第二染色体上的ade 7（45.4cM）连锁。mei 4基因与cdc 2（0.6cM）连锁在第二染色体上。基因mes 1与染色体Ⅰ上的ura 3（25.3cM）连锁。
SummaryMeiosis-deficient mutants of the fission yeast Schizosaccharomyces pombe carrying mei1, mei2, mei3, mei4 and mes1 mutant alleles were characterized by electron microscopy and staining of the nucleus with 4′, 6-diamidino-2-phenylindole. Zygotes of either mei1, mei2 or mei3 mutants contained one round nucleus with a single spindle pole body (SPB). These mutants were arrested before premeiotic DNA synthesis. Zygotes of mei4 mutants had one elongated nucleus containing thick electron-dense filaments (linear elements). In the mes1 mutant, the first meiotic division was completed but the SBPs did not duplicate. Modification of the SPB (outer plaque formation) was also blocked and the forespore membrane was not assembled.By haploidization, random spore and tetrad analyses, four essential genes for meiosis (mei2, mei3, mei4 and mes1) were mapped. Gene mei2 was located on chromosome I 14.2 cm distant from ura2. Gene mei3 was linked to ade7 (45.4 cM) on chromosome II. Gene mei4 was linked to cdc2 (0.6 cM) on chromosome II. Gene mes1 was linked to ura3 (25.3 cM) on chromosome I.