Positive correlation between CD137 expression and complex stenosis morphology in patients with acute coronary syndromes
Positive correlation between CD137 expression and complex stenosis morphology in patients with acute coronary syndromes
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DOI：
10.1016/j.cca.2011.02.038
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发表时间：
2011-05-12
影响因子：
5
通讯作者：
Chen, Guanghua
中科院分区：
文献类型：
--
作者：
Yan, Jinchuan;Gong, Jie;Chen, Guanghua
背景：CD137 是肿瘤坏死因子受体超家族的成员，据报道在急性冠状动脉综合征患者中高表达。然而，关于急性冠脉综合征患者的 CD137 表达与复杂狭窄形态之间关系的信息有限。方法：我们的研究包括正常对照 (n = 50)。稳定型心绞痛 (SA) (n = 80) 患者和急性冠脉综合征 (ACS) 患者，包括不稳定型心绞痛 (UA) (n = 70) 和急性心肌梗死 (AM!) (n = 100)。通过流式细胞术分析外周单核细胞中CD137的表达。采用酶联免疫吸附试剂盒测定血清可溶性CD137（sCD137）、MMP-9、MMP-3水平。所有直径减少>= 50%的冠状动脉狭窄均通过血管造影冠状动脉狭窄形态进行评估。结果：与对照组（8.4 +/- 2.6 MFI）和SA组（7.9 +/- 2.1）相比，ACS患者（n = 170）外周单核细胞中CD137 [23.6 +/- 5.7平均荧光强度（MFI）]表达显着增加 MFI）（p < 0.001）。 ACS 患者的 sCD137 水平（30.2 +/- 8.7 ng/ml）也高于对照组（6.2 +/- 1.8 ng/ml）和 SA 组（7.1 +/- 2.1 ng/ml）（p < 0.001）。 ACS患者血清MMP-3和MMP-9是对照组和SA组的2倍。 MMP-9之间发现正相关。外周单核细胞中 MMP-3 和 CD137 的表达以及 sCD137 水平。在可溶性或膜结合性 CD137 表达与复杂冠状动脉狭窄之间也观察到明显的相关性（r(1) = 0.5548，r(2) = 0.4652，p < 0.001）。在逻辑回归模型中，ACS 的独立预测因子为 sCD137（比值比 2.671，95% Cl 1.718-4.153，P = 0.000）、MMP-9（1.431、1.043-1.964，P = 0.026）和 MMP-3（1.368， 1.038-1.817, P = 0.018）。结论：ACS患者表现 CD137表达与复杂冠状动脉狭窄形态呈显着正相关。我们推测 CD137 表达增加可能代表或反映 ACS 患者动脉粥样硬化斑块的不稳定性。 (C) 2011 爱思唯尔 ay。版权所有。
Background: CD137, a member of the tumor necrosis factor receptor superfamily, has been reported to be expressed highly in patients with acute coronary syndromes. However, limited information is available on the relationship between CD137 expression and complex stenosis morphology in patients with acute coronary syndromes.Methods: Our study included normal controls (n = 50). patients with stable angina (SA) (n = 80) and patients with acute coronary syndromes (ACS), including unstable angina (UA) (n = 70) and acute myocardial infarction (AM!) (n = 100). The expression of CD137 in peripheral monocytes was analyzed by flow cytometry. Serum soluble CD137 (sCD137), MMP-9 and MMP-3 levels were measured by enzyme-linked immunosorbent assay kit. All coronary stenoses with >= 50% diameter reduction were assessed by angiographic coronary stenosis morphology.Results: Patients with ACS(n = 170) showed a significant increase of CD137 [23.6 +/- 5.7 mean fluorescence intensity (MFI)] expression in peripheral monocytes compared with control (8.4 +/- 2.6 MFI) and SA group (7.9 +/- 2.1 MFI) (p < 0.001). sCD137 also showed higher level in patients with ACS(30.2 +/- 8.7 ng/ml) than in control (6.2 +/- 1.8 ng/ml) and SA group (7.1 +/- 2.1 ng/ml) (p < 0.001). Serum MMP-3 and MMP-9 in patients with ACS were 2-times greater than those in control and SA group. A positive correlation was found between MMP-9. MMP-3 and CD137 expression in peripheral monocytes as well as sCD137 levels. An obvious correlation was also observed between soluble or membrane-bound CD137 expression and complex coronary stenoses (r(1) = 0.5548, r(2) = 0.4652, and p < 0.001). In the logistic regression model, the independent predictors of ACS were sCD137 (odds ratio 2.671, 95% Cl 1.718-4.153, P = 0.000), MMP-9 (1.431, 1.043-1.964, P = 0.026) and MMP-3 (1.368, 1.038-1.817, P = 0.018).Conclusion: Patients with ACS showed significantly positive correlation between CD137 expression and complex coronary stenosis morphology. We speculate that the increased CD137 expression might represent or reflect an instability of atherosclerotic plaques in patients with ACS. (C) 2011 Elsevier ay. All rights reserved.