Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C-elegans
Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C-elegans
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DOI：
10.1016/s0896-6273(00)80202-0
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发表时间：
1996-10-01
期刊：
影响因子：
16.2
通讯作者：
Ohshima, Y
中科院分区：
文献类型：
--
作者：
Komatsu, H;Mori, I;Ohshima, Y
梭秀丽线虫Tax-4突变体在多种感觉行为中是异常的：它们不能对温度或水溶性或挥发性化学引诱剂作出反应。我们表明，预测的税收-4基因产物是高度同源的脊椎动物环核苷酸门控通道。在培养细胞中表达的Tax-4蛋白作为环核苷酸门控通道发挥功能。绿色荧光蛋白（GFP）标记的功能性Tax-4蛋白在热敏、味觉和嗅觉神经元中表达，介导受Tax-4突变影响的所有感觉行为。Tax 4：：GFP融合部分位于这些神经元的感觉末梢。结果表明，环核苷酸门控通道是温度感觉和化学感觉所必需的，cGMP是C.感觉传导
The C. elegans tax-4 mutants are abnormal in multiple sensory behaviors: they fail to respond to temperature or to water-soluble or volatile chemical attractants. We show that the predicted tax-4 gene product is highly homologous to vertebrate cyclic nucleotide-gated channels. Tax-4 protein expressed in cultured cells functions as a cyclic nucleotide-gated channel. The green fluorescent protein (GFP)-tagged functional Tax-4 protein is expressed in thermosensory, gustatory, and olfactory neurons mediating all the sensory behaviors affected by the tax-4 mutations. The Tax4::GFP fusion is partly localized at the sensory endings of these neurons. The results suggest that a cyclic nucleotide-gated channel is required for thermosensation and chemosensation and that cGMP is an important intracellular messenger in C. elegans sensory transduction.