CHARACTERIZATION OF A CHITINASE GENE (CHIA) FROM SERRATIA-MARCESCENS BJL200 AND ONE-STEP PURIFICATION OF THE GENE-PRODUCT
CHARACTERIZATION OF A CHITINASE GENE (CHIA) FROM SERRATIA-MARCESCENS BJL200 AND ONE-STEP PURIFICATION OF THE GENE-PRODUCT
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DOI：
10.1016/0378-1097(94)00462-5
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发表时间：
1994-12-15
影响因子：
2.1
通讯作者：
NES, IF
中科院分区：
文献类型：
--
作者：
BRURBERG, MB;EIJSINK, VGH;NES, IF
测定了粘质沙雷氏菌菌株BJL200的chiA基因的核苷酸序列。该基因被发现编码一个由 563 个氨基酸残基组成的蛋白质，具有一个典型的由 23 个残基组成的 N 端信号肽，在输出过程中被切割掉。该基因在对应于基因产物的氨基酸残基410-467的区域中表现出与先前表征的粘质链球菌的两个chiA基因的显着差异。含有克隆基因的大肠杆菌菌株的周质级分被用作开发基于疏水相互作用色谱的几丁质酶的快速、一步纯化方案的起始材料。
The nucleotide sequence of the chiA gene from Serratia marcescens strain BJL200 was determined. The gene was found to encode a protein of 563 amino acid residues, with a typical N-terminal signal peptide of 23 residues, that is cleaved off during export. The gene exhibited striking differences with two previously characterized chiA genes of S. marcescens in the region corresponding to amino acid residues 410-467 of the gene product. Periplasmic fractions of an Escherichia coli strain harbouring the cloned gene were used as starting material for the development of a fast, one-step purification protocol for the chitinase that is based on hydrophobic interaction chromatography.