Engineering Zymomonas mobilis for robust cellulosic ethanol production
Engineering Zymomonas mobilis for robust cellulosic ethanol production
复制标题
改造运动发酵单胞菌以实现稳健的纤维素乙醇生产
DOI：
10.1016/j.tibtech.2019.02.002
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发表时间：
2019
影响因子：
17.3
通讯作者：
Bai Feng-Wu
中科院分区：
文献类型：
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作者：
Xia Juan;Yang Yongfu;Liu Chen-Guang;Yang Shihui;Bai Feng-Wu
利用氧化还原酶途径对戊糖代谢的酿酒酵母进行了大量的研究，但存在内在的辅因子失衡问题，这严重影响了乙醇的产量。运动发酵单胞菌不仅可以通过异构酶途径代谢戊糖而没有辅因子失衡，而且可以通过Entner-Doudoroff途径代谢糖，以较少的三磷酸腺苷和生物量产生更多的糖用于乙醇生产。此外，还讨论了多个Z的基因组序列信息的可用性。移动菌和先进的遗传学工具为该物种的工程奠定了坚实的基础，而细菌细胞的自絮凝特性也为生物过程工程提供了显著的优势。在这里，我们重点介绍这些方面的一些最新进展。
Great effort has been devoted to engineeringSaccharomyces cerevisiaewith pentose metabolism through the oxido-reductase pathway for cellulosic ethanol production, but intrinsic cofactor imbalance is observed, which substantially compromises ethanol yield.Zymomonas mobilisnot only can be engineered for pentose metabolism through the isomerase pathway without cofactor imbalance but also metabolizes sugar through the Entner–Doudoroff pathway with less ATP and biomass produced for more sugar to be used for ethanol production. Moreover, the availabilities of genome sequence information for multipleZ. mobilisstrains and advanced genetics tools have laid a solid foundation for engineering this species, and the self-flocculation of the bacterial cells also presents significant advantages for bioprocess engineering. Here, we highlight some of recent advances in these aspects.