Mannose7 glycan isomer characterization by IMS-MS/MS analysis.
Mannose7 glycan isomer characterization by IMS-MS/MS analysis.
复制标题
通过 IMS-MS/MS 分析进行甘露糖 7 聚糖异构体表征。
DOI：
10.1007/s13361-012-0491-y
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发表时间：
2012
影响因子：
3.2
通讯作者：
Clemmer,DavidE
中科院分区：
文献类型：
--
作者：
Zhu,Feifei;Lee,Sunyoung;Valentine,StephenJ;Reilly,JamesP;Clemmer,DavidE
用离子迁移谱-串联质谱（IMS-MS/MS）对牛核糖核酸酶B中man7glcnac2聚糖的异构体进行了表征。在这些实验中，通过离子迁移谱法选择具有不同迁移率的[Man7 + 2Na]2+前驱体，并在离子阱中使用MS/MS技术进行分析。得到的不同前体离子的破碎谱具有特异性，表明分离或富集了不同的聚糖异构体。发现一个质荷比（m/z）为903.8的碎片离子对应于特定异构体内部甘露糖残基的损失。提取的碎片离子漂移时间分布（XFIDTDs）产生独特的前体离子漂移时间分布，表明存在四种不同的异构体。
The isomers of the Man7GlcNAc2glycan obtained from bovine ribonuclease B have been characterized by ion mobility spectrometry-tandem mass spectrometry (IMS-MS/MS). In these experiments, [Man7 + 2Na]2+precursors having different mobilities are selected by ion mobility spectrometry and analyzed by MS/MS techniques in an ion trap. The fragmentation spectra obtained for various precursor ions are specific, suggesting the isolation or enrichment of different glycan isomers. One fragment ion with a mass-to-charge ratio (m/z) of 903.8 is found to correspond to the loss of an internal mannose residue of a specific isomer. Extracted fragment ion drift time distributions (XFIDTDs) yield distinctive precursor ion drift time profiles indicating the existence of four separate isomers as proposed previously.