Effect of deglycosylation on the subunit interactions and receptor binding activity of human chorionic gonadotropin.
Effect of deglycosylation on the subunit interactions and receptor binding activity of human chorionic gonadotropin.
复制标题
去糖基化对人绒毛膜促性腺激素亚基相互作用和受体结合活性的影响。
DOI：
10.1016/s0006-291x(81)80145-3
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发表时间：
1981
影响因子：
3.1
通讯作者：
O. P. Bahl
中科院分区：
文献类型：
--
作者：
N. Kalyan;O. P. Bahl
碳水化合物在亚基相互作用和与受体结合中的作用通过用三氟甲磺酸（TFMS）在0°下使hCG的单个α-和β-亚基去糖基化5 h来评估。TFMS处理分别去除hCG-α和hCG-β亚基中约90%和81%的碳水化合物，而不影响蛋白质核心，如通过SDS-凝胶电泳测定的氨基酸分析、免疫活性和分子量所证明的。去糖基化的亚基不仅能够完全重新结合，如重构的去糖基化的hCG的氨基酸组成所示，而且恢复了与天然hCG相当的免疫学和受体结合特性（90-105%）。上述数据有力地表明，两个亚基中的碳水化合物在亚基的相互作用和激素的受体结合活性中是不需要的。
The role of carbohydrate in the subunit interactions and in the binding to the receptor was assessed by the deglycosylation of the individual α- and β-subunits of hCG with trifluoromethane sulfonic acid (TFMS) at 0° for 5 h. The treatment with TFMS removed about 90% and 81% of the carbohydrates from hCG-α and hCG-β subunits respectively, without affecting the protein core as evidenced by their amino acid analyses, immunological activities and molecular weights, determined by SDS-gel electrophoresis. The deglycosylated subunits not only were able to reassociate completely as shown by the amino acid composition of the reconstituted deglycosylated hCG, but also recovered their immunological and receptor binding properties (90–105%) comparable to the native hCG. The above data strongly suggest that the carbohydrates in both subunits are not required in the interaction of the subunits and in the receptor binding activity of the hormone.