Regulation of hepatic cholesterol biosynthesis by fatty acids: effect of feeding olive oil on cytoplasmic acetoacetyl-coenzyme A thiolase, beta-hydroxy-beta-methylglutaryl-CoA synthase, and acetoacetyl-coenzyme A ligase.
Regulation of hepatic cholesterol biosynthesis by fatty acids: effect of feeding olive oil on cytoplasmic acetoacetyl-coenzyme A thiolase, beta-hydroxy-beta-methylglutaryl-CoA synthase, and acetoacetyl-coenzyme A ligase.
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脂肪酸对肝胆固醇生物合成的调节：饲喂橄榄油对细胞质乙酰乙酰辅酶 A 硫解酶、β-羟基-β-甲基戊二酰辅酶 A 合酶和乙酰乙酰辅酶 A 连接酶的影响。
DOI：
10.1016/s0006-291x(88)81241-5
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发表时间：
1988
影响因子：
3.1
通讯作者：
Heimberg,M
中科院分区：
文献类型：
--
作者：
Salam,WH;Cagen,LM;Heimberg,M
我们之前曾报道，在灌流的大鼠肝脏中，油酸增加了胆固醇生物合成的胞浆酶的比活性。在本研究中，我们研究了口服橄榄油对HMG-CoA合成酶、acac-CoA硫解酶、acac-CoA连接酶和HMG-CoA还原酶活性的影响。饲喂橄榄油可使肝脏HMG-CoA合酶比活力提高50%，Acac-CoA硫解酶比活力提高2倍，Acac-CoA连接酶比活力提高3倍。橄榄油对HMG-CoA还原酶活性无影响。这些数据表明，在这些条件下，提供肝脏胆固醇生成所需的HMG-CoA的酶是由一种生理底物脂肪酸平行调节的，而不是HMG-CoA还原酶。
We reported previously that, in the perfused rat liver, oleic acid increased the specific activity of cytosolic enzymes of cholesterol biosynthesis. In this study, we examined the effects of oral administration of olive oil on the activities of HMG-CoA synthase, AcAc-CoA thiolase, AcAc-CoA ligase and HMG-CoA reductase. Olive oil feeding increased the specific activity of hepatic HMG-CoA synthase by 50%, AcAc-CoA thiolase by 2-fold, and AcAc-CoA ligase by 3-fold. Olive oil had no effect on HMG-CoA reductase activity. These data suggest that the enzymes that supply the HMG-CoA required for hepatic cholesterogenesis are regulated in parallel by a physiological substrate, fatty acid, independent of HMG-CoA reductase under these conditions.