The effect of the cytoplasmic tail of influenza C virus CM2 protein on its biochemical properties and intracellular processing.
The effect of the cytoplasmic tail of influenza C virus CM2 protein on its biochemical properties and intracellular processing.
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DOI：
10.1016/j.bbrep.2015.07.001
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发表时间：
2015-09
影响因子：
2.7
通讯作者：
Hongo S
中科院分区：
文献类型：
--
作者：
Shimotai Y;Goto T;Matsuzaki Y;Muraki Y;Sugawara K;Hongo S
CM 2是由丙型流感病毒M基因编码的整合膜蛋白。为了研究CM 2胞质尾区对其生化特性的影响，在CM 2胞质尾区47-115位残基处引入缺失和取代突变，并对表达的CM 2突变体进行研究。虽然胞质尾区对于CM 2的寡聚化不是必需的，但它可能影响寡聚化的程度。残基47-48、67-69、73-90和113-115都是CM 2的适当表达所需的。脉冲追踪实验表明，残基47-48、67-69、73-75和79-87稳定CM 2，从而影响CM 2表达。在残基61-115处的C-末端区域对于CM 2转运至细胞表面不是必需的，并且14个氨基酸而不是11个氨基酸的胞质尾区对于CM 2的细胞表面表达是足够的。这些结果表明，某些氨基酸序列或CM 2胞质尾的长度是必要的适当的构象成熟，稳定性，表达水平和胞内运输的CM 2。胞质尾区调节CM 2寡聚化的效率。残基47-48、67-69、73-90和113-115是CM 2的适当表达所需的。残基47-48、67-69、73-75和79-87稳定CM 2。胞质14个氨基酸足以用于CM 2的细胞表面表达。
CM2 is an integral membrane protein encoded by the influenza C virus M gene. To examine the effects of the cytoplasmic tail of CM2 on its biochemical properties, deletion and substitution mutations were introduced into CM2 cytoplasmic tail at residues 47–115, and the expressed CM2 mutants were investigated. Although the cytoplasmic tail is not essential for the oligomerization of CM2, it may affect the degree of oligomerization. The residues 47–48, 67–69, 73–90 and 113–115 were all required for the proper expression of CM2. Pulse-chase experiments suggest that residues 47–48, 67–69, 73–75 and 79–87 stabilize CM2, thereby affecting CM2 expression. The C-terminal region at residues 61–115 is not essential for CM2 transport to the cell surface, and a 14-amino-acid, but not an 11-amino-acid, cytoplasmic tail is sufficient for the cell surface expression of CM2. These results suggest that either certain amino acid sequences or the length of the CM2 cytoplasmic tail are necessary for the proper conformational maturation, stability, expression level and intracellular transport of CM2. The cytoplasmic tail modulates the efficiency of CM2 oligomerization. The residues 47–48, 67–69, 73–90 and 113–115 are required for the proper expression of CM2. The residues 47–48, 67–69, 73–75 and 79–87 stabilize CM2. Cytoplasmic 14 amino acids are sufficient for the cell surface expression of CM2.