Evidence for the expression of alcohol dehydrogenase class I gene in rat uterus and its up-regulation by progesterone.
Evidence for the expression of alcohol dehydrogenase class I gene in rat uterus and its up-regulation by progesterone.
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DOI：
10.1507/endocrj.k07-082
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发表时间：
2008-01
影响因子：
2
通讯作者：
T. Ohno;H. Hiroi;M. Momoeda;Yumi Hosokawa;R. Tsutsumi;M. Koizumi;F. Nakazawa;T. Yano;O. Tsutsumi;Y. Taketani
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文献类型：
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作者：
T. Ohno;H. Hiroi;M. Momoeda;Yumi Hosokawa;R. Tsutsumi;M. Koizumi;F. Nakazawa;T. Yano;O. Tsutsumi;Y. Taketani
子宫内膜是卵巢类固醇激素、雌激素和孕激素的靶组织之一。为了阐明子宫内膜基因调控的机制，采用抑制性消减杂交技术分离了大鼠子宫中孕酮调控的候选基因。乙醇脱氢酶(ADH)I类基因是候选基因之一。在此，我们研究了ADH I类基因在大鼠子宫中的表达和调控。采用RT-PCR方法检测子宫组织中ADH-I类基因的表达。用ADH-I类特异性探针进行原位杂交，研究ADH-I在大鼠子宫中的定位。原位杂交在大鼠子宫内膜间质细胞中检测到阳性信号，在子宫内膜上皮细胞和子宫肌层中未检测到阳性信号。用17-β雌二醇和孕酮处理去卵巢大鼠，取子宫进行Northern印迹分析和ADH活性测定。Northern印迹分析显示，孕酮处理后大鼠子宫中ADH I类基因的表达上调了近2倍，而雌激素处理后子宫中ADH I类基因的表达没有上调。同样，黄体酮处理的大鼠子宫中ADH活性大约是对照组的两倍。这项研究证明ADH I类基因在子宫中是孕酮反应的。这意味着孕酮可能与子宫中维甲酸的合成有关，因为ADH是维甲酸合成的关键酶。
The endometrium is one of the target tissues of the ovarian steroid hormones, estrogen and progesterone. In order to elucidate the mechanism of gene regulation in the endometrium, suppressive subtraction hybridization was performed to isolate the candidate genes controlled by progesterone in rat uterus. Alcohol dehydrogenase (ADH) class I gene was one of the candidate genes. Here we investigated the expression and regulation of ADH class I gene in rat uterus. The mRNA of ADH class I was detected in uterus by RT-PCR using specific primers. Using specific probe for ADH class I, in situ hybridization was performed to investigate localization in rat uterus. Positive signals were detected in the endometrial stromal cells of rat uterus by in situ hybridization and were not detected in endometrial epithelial cells and myometrium in rat uterus. Ovariectomized rats were treated with 17-beta estradiol and progesterone and the uteri of these rats were used for Northern blot analysis and assay of the ADH activity. Northern blot analysis revealed that the expression of ADH class I mRNA in rat uteri was up-regulated approximately two-fold after progesterone treatment, but not estrogen. Likewise, ADH activity was approximately two-fold higher in progesterone-treated rat uteri compared with controls. This study demonstrated that ADH class I gene is progesterone-responsive in the uterus. This implies that progesterone might be involved with retinoic acid synthesis in the uterus, since ADH is the key enzyme for retinoic acid synthesis.