DETECTION OF INFECTIOUS-BRONCHITIS VIRUS-ANTIGEN FROM EXPERIMENTALLY INFECTED CHICKENS BY INDIRECT IMMUNOFLUORESCENT ASSAY WITH MONOCLONAL-ANTIBODY
DETECTION OF INFECTIOUS-BRONCHITIS VIRUS-ANTIGEN FROM EXPERIMENTALLY INFECTED CHICKENS BY INDIRECT IMMUNOFLUORESCENT ASSAY WITH MONOCLONAL-ANTIBODY
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DOI：
10.2307/1591405
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发表时间：
1990-04-01
期刊：
影响因子：
1.4
通讯作者：
OHTA, S
中科院分区：
文献类型：
--
作者：
YAGYU, K;OHTA, S
用单克隆抗体（MAb-IFA）间接免疫荧光法检测鸡传染性支气管炎病毒（IBV）。该单克隆抗体对IBV M41株核蛋白具有特异性。单克隆抗体-间接免疫荧光法在感染的鸡肾细胞中清楚地检测到IBV，具有高度特异性。该检测方法还检测到实验感染鸡的气管涂片和切片气管中的IBV。发现阳性反应时间长于病毒恢复试验。结果表明，单克隆抗体-间接免疫荧光法是一种检测疑似传染性支气管炎鸡群中IBV的有效方法。
Infectious bronchitis virus (IBV) was detected by indirect immunofluorescent assay with a monoclonal antibody (MAb-IFA). The monoclonal antibody was specific for the nucleocapsid protein of IBV strain M41. The MAb-IFA clearly detected IBV with high specificity in infected chicken kidney cells. The assay furthermore detected IBV in tracheal smears and sliced tracheas from experimentally infected chickens. The positive reaction was found to be longer than that in the virus recovery test. These results indicate that MAb-IFA is a useful method for the detection of IBV from chickens suspected to have infectious bronchitis.