Newly breeding an inbred strain of ischemia-prone Mongolian gerbils and its reproduction and genetic characteristics.
Newly breeding an inbred strain of ischemia-prone Mongolian gerbils and its reproduction and genetic characteristics.
复制标题
缺血性沙鼠近交系新选育及其繁殖和遗传特性
DOI：
10.1538/expanim.17-0071
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发表时间：
2018-02-09
影响因子：
2.4
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Du X;Wang D;Li Y;Huo X;Li C;Lu J;Wang Y;Guo M;Chen Z
蒙古沙土鼠在许多研究领域都是有用的实验动物，特别是在缺血研究中。然而，由于Willis环(CoW)的变化，所建立的缺血模型是不稳定和多样的。为了解决这个问题，我们新建立了一个近交系沙土鼠，限制繁殖并保持到F23。本研究描述了这些动物的繁殖和生长数据。用微卫星DNA和生化标记检测F4~F20的遗传特性。结果表明，单侧颈总动脉结扎造成的缺血模型和不完全COW的发生率均增加，分别由F1的50%和75%增加到F20的88.89%和100%。双亲母牛一致模式的比例与近交世代数呈正相关。繁殖性能分析表明，近交系长爪沙鼠的平均产仔数小于近交系长爪沙鼠，成年体重也低于近交系长爪沙鼠，且选择第2胎的幼鼠繁殖效果最好。遗传检测结果表明，在F20的28个微卫星座位和26个生化标记中，有26个是纯合的，表明近交系沙土鼠的遗传组成基本相同。这些数据表明，已成功建立了一个近交系的缺血易感沙土鼠。这种菌株可以用于中风研究，并可以极大地减少实验所需的动物数量。
The Mongolian gerbil has been a useful laboratory animal in many research fields, especially in ischemia studies. However, due to the variation of the circle of Willis (COW), the ischemic model is unstable and various. To solve this problem, we newly established an inbred strain of gerbils, restricting breeding and keeping to F23. The data on the breeding and growth of the animals are described in the present study. The genetic characteristics of F4 to F20 detected by microsatellite DNA and biochemical markers are also shown here. The results demonstrated that the frequency of ischemic model by unilateral carotid occlusion and the frequency of incomplete COW increased, increasing from 50% and 75% in F1 to 88.89% and 100% in F20, respectively. The ratios of consistent patterns of COW in parents were positively related with the number of inbred generations. A reproductive performance analysis indicated that the average size of litters in the inbred gerbils was less than that of outbred gerbils and that adult body weight was also lower in inbred gerbils; also, the pups in the 2nd litter were the best ones chosen to reproduce. The genetic detection results indicated that 26 out of 28 microsatellite loci and all 26 biochemical markers were homozygous in F20, showing comparably identical genetic composition in inbred gerbils. All the data demonstrated that an inbred strain of ischemia-prone gerbil has been established successfully. This strain can be used in stroke research and can largely reduce the number of animals needed in experiments.