Automatable production of shippable bilayer chips by pin tool deposition for an ion channel measurement platform.
Automatable production of shippable bilayer chips by pin tool deposition for an ion channel measurement platform.
复制标题
通过离子通道测量平台的针工具沉积自动生产可运输的双层芯片。
DOI：
10.1002/biot.200900297
复制
发表时间：
2010
影响因子：
4.7
通讯作者：
Schmidt,JacobJ
中科院分区：
文献类型：
--
作者：
Poulos,JasonL;Jeon,Tae-Joon;Schmidt,JacobJ
作为迈向自动化且无需操作员的离子通道测量平台的一步，我们之前已经展示了一种人工脂质双层的解决方案配方，该配方能够无限期储存和运输冷冻双层前体。在这项工作中，溶液是手工沉积的。在这里，我们采用了针工具将双层前体溶液沉积到多元件阵列上，这是微阵列溶液沉积的一种流行方法。针工具使沉积体积能够高度重复和可控地施加，从而将双层形成时间缩短至 <1 小时。销钉工具还与计算机化运动控制平台兼容，从而实现自动化和高产量生产。我们讨论了这些结果以及该技术的前景，以生产高密度双层阵列，用于对人工双层中的离子通道进行高通量测量。
As a step towards an automated and operator‐free ion channel measurement platform we have previously demonstrated a solution formulation for artificial lipid bilayers that enabled the indefinite storage and shipping of frozen bilayer precursors. In this work, the solutions were deposited by hand. Here, we have adapted pin tools to deposit the bilayer precursor solutions onto multi‐element arrays, a popular method for microarray solution deposition. The pin tools have enabled the deposited volume to be applied highly repeatably and controllably, resulting in reduction of bilayer formation times to <1 h. The pin tools are also compatible with computerized motion control platforms, enabling automated and high throughput production. We discuss these results and the prospects of this technology to produce high density bilayer arrays for high throughput measurement of ion channels incorporated into artificial bilayers.