EFFECTS OF METAL-IONS, INCLUDING MG2+ AND LANTHANIDES, ON THE CLEAVAGE OF RIBONUCLEOTIDES AND RNA MODEL COMPOUNDS
EFFECTS OF METAL-IONS, INCLUDING MG2+ AND LANTHANIDES, ON THE CLEAVAGE OF RIBONUCLEOTIDES AND RNA MODEL COMPOUNDS
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DOI：
10.1073/pnas.88.10.4080
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发表时间：
1991-05-01
影响因子：
11.1
通讯作者：
HUANG, DL
中科院分区：
文献类型：
--
作者：
BRESLOW, R;HUANG, DL
Eu ~（3+）对3 ′，5 ″-尿苷基尿苷的环化/裂解反应有很强的催化作用，Eu ~（3+）、Tb ~（3+）和Yb ~（3+）对1-对硝基苯基磷酸丙二醇酯的环化/裂解反应也有很强的催化作用。Mg ~（2+）对该反应有一定的催化作用，Ca ~（2+）对该反应无催化作用; Zn ~（2+）和Pb ~（2+）对该反应也有较好的催化作用。各种配体进一步激活这些反应，咪唑显然作为一个额外的碱催化剂。一些环糊精衍生物的作用是结合底物和金属离子，但与其他地方报道的相反，核苷酸之间没有强的选择性，这可以归因于环糊精结合。
The cyclization/cleavage of 3',5"-uridyluridine to form 2',3'-cyclic uridylic acid is very effectively catalyzed by Eu3+, and the cyclization/cleavage of the 1-p-nitrophenyl phosphate ester of propane-1,2-diol also shows strong metal ion catalysis by Eu3+, Tb3+, and Yb3+. It also shows moderate catalysis by Mg2+, but not by Ca2+; Zn2+ and Pb2+ are also good catalysts. Various ligands activate these reactions further, and imidazole apparently acts as an additional base catalyst. Some cyclodextrin derivatives act to bind both the substrate and the metal ion but, contrary to what is reported elsewhere, there is no strong selectivity among nucleotides that can be ascribed to cyclodextrin binding.