Imaging the developing lymphatic system using the zebrafish.
Imaging the developing lymphatic system using the zebrafish.
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DOI：
10.1002/9780470319413.ch11
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发表时间：
2007-09
影响因子：
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通讯作者：
K. Yaniv;Sumio Isogai;Daniel Castranova;Louis Dye;J. Hitomi;B. Weinstein
中科院分区：
文献类型：
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作者：
K. Yaniv;Sumio Isogai;Daniel Castranova;Louis Dye;J. Hitomi;B. Weinstein
淋巴系统对于免疫反应、液体稳态和脂肪吸收至关重要，并且参与许多病理过程，包括肿瘤转移和淋巴水肿。尽管它很重要，但由于观察体内淋巴细胞以及对淋巴系统进行遗传和实验操作的困难，理解该系统的起源和早期发展的进展受到阻碍。这些困难部分源于缺乏结合这些特征的模式生物。斑马鱼是一种基因可及的脊椎动物，具有光学透明的胚胎，可以进行高分辨率的体内成像，但此前尚未报道过该模型生物中存在淋巴管系统。通过一系列形态学、分子和功能研究，我们对发育中的斑马鱼的淋巴管进行了可视化和表征。我们的研究表明，斑马鱼拥有的淋巴系统与其他脊椎动物的淋巴管具有许多共同特征。使用多光子延时成像，我们进行了体内细胞追踪实验，以追踪淋巴管内皮细胞的起源。我们的数据提供了确凿的新证据，支持原始淋巴内皮细胞的静脉起源。
The lymphatic system is essential for immune responses, fluid homeostasis, and fat absorption, and is involved in many pathological processes, including tumour metastasis and lymphoedema. Despite its importance, progress in understanding the origins and early development of this system has been hampered by difficulties in observing lymphatic cells in vivo and performing genetic and experimental manipulation of the lymphatic system. These difficulties stem in part from the lack of a model organism combining these features. The zebrafish is a genetically accessible vertebrate with an optically clear embryo permitting high-resolution in vivo imaging, but the existence of a lymphatic vascular system has not been previously reported in this model organism. Using a series of morphological, molecular and functional studies we have visualized and characterized lymphatic vessels in the developing zebrafish. Our studies show that the zebrafish possesses a lymphatic system that shares many of the characteristic features of lymphatic vessels found in other vertebrates. Using multiphoton time-lapse imaging we have carried out in vivo cell tracking experiments to trace the origins of lymphatic endothelial cells. Our data provide conclusive new evidence supporting a venous origin for primitive lymphatic endothelial cells.