ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines
ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines
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ST13 是一种增殖调节剂，可抑制结直肠癌细胞系的生长和迁移
DOI：
10.1631/jzus.b1200037
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发表时间：
2012-11-01
影响因子：
5.1
通讯作者：
Zheng, Shu
中科院分区：
文献类型：
--
作者：
Bai, Rui;Shi, Zhong;Zheng, Shu
背景与目的ST 13基因是编码热休克蛋白70相互作用蛋白（HIP）的基因。先前的研究表明，与邻近正常组织相比，结直肠癌（CRC）组织中ST 13 mRNA和蛋白水平下调。本研究旨在探讨ST 13在结直肠癌细胞增殖和迁移中的作用。分别通过慢病毒转染法构建ST 13过表达和ST 13敲减的大肠癌细胞株，通过MTT法、平板集落形成法、细胞周期分析和迁移实验评价ST 13对大肠癌细胞体外增殖和迁移的影响。此外，小鼠异种移植研究进行测试在体内致瘤性的ST 13敲低CRC cells.ResultsLentivirus-mediated过表达的ST 13在CRC细胞抑制细胞增殖，集落形成，和细胞迁移在体外。相反，通过基于慢病毒的短发夹RNA（shRNA）干扰在CRC细胞中下调ST 13显著增加了体外细胞增殖和克隆效率。此外，ST 13表达下调可显着增加结直肠癌细胞的体内致瘤性。结论ST 13基因是一种增殖调节因子，可抑制结直肠癌肿瘤的生长，并可能影响细胞迁移。
Background and objectiveST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells.MethodsThe transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells.ResultsLentivirus-mediated overexpression of ST13 in CRC cells inhibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviral-based short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo.ConclusionsST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration.