Increased trehalase expression after glucose limitation of LLC-PK1 clones.
Increased trehalase expression after glucose limitation of LLC-PK1 clones.
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LLC-PK1 克隆的葡萄糖限制后海藻糖酶表达增加。
DOI：
10.1152/ajpcell.1988.255.6.c816
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Lever,JE
中科院分区：
文献类型：
--
作者：
Yoneyama,Y;Lever,JE
在来源于肾上皮的LLC-PK1细胞系中，顶膜二糖酶海藻糖酶的表达与近端小管特异性分化表型的发展相关。在融合培养体系中，用半乳糖代替葡萄糖，海藻糖酶的诱导效果显著；用果糖、甘露糖或非代谢糖α-甲基-D-葡萄糖代替后，海藻糖酶的诱导作用较弱。在其他微绒毛水解酶中，海藻糖酶的诱导是特异的，替代葡萄糖后是可逆的，并且随着葡萄糖浓度的降低而增加。非选择性地分离葡萄糖限制诱导和非诱导克隆的稳定克隆细胞系。在分化诱导剂N，N‘-六亚甲基双乙酰胺(HMBA)作用下，两种细胞均表现出海藻糖酶的诱导作用。这些结果表明，缺糖后海藻糖酶的诱导机制不同于HMBA介导的机制。
Expression of the apical membrane disaccharidase trehalase correlates with the development of a proximal tubule-specific differentiated phenotype in the LLC-PK1 cell line derived from renal epithelia. Substitution of galactose for glucose in the culture medium of confluent cultures resulted in a significant trehalase induction; a smaller induction was observed after replacement by fructose, mannose, or the nonmetabolizable sugar alpha-methyl-D-glucose. Induction was specific for trehalase among other microvillar hydrolases, reversible after replacement of glucose, and was increased as a function of decreasing glucose concentration. Stable clonal cell lines that were inducible by glucose limitation and noninducible clones were isolated nonselectively. Both types exhibited trehalase induction after exposure to the differentiation inducer N,N'-hexamethylene-bis-acetamide (HMBA). These results suggest that trehalase induction after glucose deprivation occurs by a different mechanism than that mediated by HMBA.