Studies on retinal capillary cells in tissue culture.
Studies on retinal capillary cells in tissue culture.
复制标题
组织培养中视网膜毛细血管细胞的研究。
DOI：
10.1016/0042-6989(81)90154-1
复制
发表时间：
1981
期刊：
影响因子：
1.8
通讯作者：
Frank,RN
中科院分区：
文献类型：
--
作者：
Frank,RN
在适当的条件下，可以从几个物种的视网膜毛细血管中培养出单独的细胞类型。通过匀浆-过滤技术分离成年动物的毛细血管，然后将其放入培养液中，单层细胞增殖，可被鉴定为来自壁内周细胞。到目前为止，鉴定主要是基于原始血管外植体的放射自显影。当将视网膜血管系统不成熟的胎儿或幼年动物的视网膜毛细血管置于培养中时，会产生一层缓慢生长的单层内皮细胞。这里的鉴定是基于细胞的光镜形态和电子显微镜下发现的小带闭塞，并在适当物种的制剂中，通过因子免疫荧光和血管紧张素转换酶活性进行鉴定。到目前为止，这些血管细胞培养已经用于研究醛糖还原酶系统和胶原合成，这两者都是可能与糖尿病视网膜病变的发病机制无关的生化机制。对于进一步研究视网膜最小血管的生化、生理学和生长潜力，个别类型的视网膜毛细血管培养的可用性应该是一种非常有用的方法。
Under appropriate conditions, it is possible to culture individual cell types from the retinal capillaries of several species. When capillaries from adult animals are isolated by a homogenization-filtration technique, and then placed in culture medium, a monolayer of cells proliferate that are identifiable as deriving from the intramural pericytes. Thus far, identification is based largely on radioautography of the original vessel explants. When capillaries from the retinas of fetal or very young animals with immature retinal vascular systems are placed in culture, a slowly growing monolayer of endothelial cells results. Identification here is based on the light microscopic morphology of the cells together with the finding zonulae occludentes by electron microscopy, and, in preparations from appropriate species, by factor VIII immunofluorescence and angiotensin converting enzyme activity. In preliminary investigations to date, these vascular cell cultures have been used in the studies of the aldose reductase system and of collagen synthesis, both of which are biochemical mechanisms that may be irrelevant to the pathogenesis of diabetic retinopathy. The availability of cultures of individual types of retinal capillary cells should be an extremely useful method for further studies of the biochemistry, physiology, and growth potential of the smallest blood vessels of the retina.