ARTIFACTS ASSOCIATED WITH QUICK-FREEZING AND FREEZE-DRYING
ARTIFACTS ASSOCIATED WITH QUICK-FREEZING AND FREEZE-DRYING
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DOI：
10.1016/s0022-5320(83)90047-3
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
LASSIGNAL, NL
中科院分区：
文献类型：
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作者：
MILLER, KR;PRESCOTT, CS;LASSIGNAL, NL
非生物样品在液氦冷却的冷冻冲击装置中进行速冻和冷冻干燥时产生的结构。以这种方式检查的样品包括NaCl、蔗糖和Tris [三（羟甲基）氨基甲烷]缓冲液。在这些制剂中形成了多种骨小梁样和骨小梁样结构。这些结构可以代表在冷冻过程期间小冰晶生长期间形成的低共熔混合物，并且在蚀刻过程期间纯冰快速升华期间暴露。通过这种技术，在各种缓冲液中制备生物膜样品（分离的[菠菜]叶绿体膜）。在蒸馏水中，获得了膜表面的优异复制品。在盐溶液中，膜似乎嵌入在一个非常像细胞骨架晶格的细丝网络中。当采用这种制备技术研究EM的细胞结构时，应特别小心，可能需要在蒸馏水中对细胞组分进行大量洗涤，以获得细胞结构的忠实表现。
The structures produced when nonbiological samples were subjected to quick-freezing and freeze-drying with a liquid He cooled freeze-slamming device. Samples examined in this way included NaCl, sucrose, and Tris [tris (hydroxymethyl) aminomethane] buffer. A variety of filamentlike and trabeculumlike structures were formed in these preparations. These structures may represent eutectic mixtures formed during the growth of small ice crystals during the freezing process, and exposed during the rapid sublimation of pure ice during the etching process. Samples of biological membranes (isolated [spinach] chloroplast membranes) were prepared in various buffers by means of this technique. In distilled water, excellent replicas of membrane surfaces were obtained. In salt solutions the membranes appeared to be embedded in a network of thin filaments appearing very much like a cytoskeletal lattice. Extreme caution should be used when employing this preparation technique for studies of cell architecture for EM extensive washing of cell components in distilled water may be necessary to obtain faithful representations of cell structure.