Trimming of MHC Class I Ligands by ERAP Aminopeptidases.
Trimming of MHC Class I Ligands by ERAP Aminopeptidases.
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通过 ERAP 氨基肽酶修剪 MHC I 类配体。
DOI：
10.1007/978-1-4939-9450-2_3
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Bouvier,Marlene
中科院分区：
文献类型：
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作者：
Weimershaus,Mirjana;Evnouchidou,Irini;Li,Lenong;vanEndert,Peter;Bouvier,Marlene
在过去的十年中，对主要组织相容性复合体（MHC）I类抗原呈递途径的表征的研究已经强调了抗原加工、肽转运、肽修整和肽选择作为开发由MHC I类分子呈递给细胞毒性T淋巴细胞（CTL）的最佳肽库的关键阶段的重要性。这些阶段的研究以及它们是如何调节的，对于理解适应性免疫系统的进展至关重要。在这里，我们描述了一种体外测定监测肽修剪的人内质网氨基肽酶1（ERAP 1）和ERAP 2（ERAP）作为一种工具，以表征修剪事件，并获得更好地了解ERAP在肽库开发的作用和功能。具体而言，我们的测定允许监测游离肽的修整，也可以监测MHC I结合肽的修整，这可以最好地反映生理情况。
Studies over the last decade on characterization of the major histocompatibility complex (MHC) class I antigen presentation pathway have highlighted the importance of antigen processing, peptide transport, peptide trimming, and peptide selection as key stages for the development of optimal peptide repertoires that are presented by MHC class I molecules to cytotoxic T lymphocytes (CTLs). The study of these stages and how they are regulated, is fundamental for progress in understanding the adaptive immune system. Here we describe an in vitro assay monitoring peptide trimming by the human endoplasmic reticulum amino peptidases 1 (ERAP1) and ERAP2 (ERAPs) as a tool to characterize trimming events and gain a better understanding of the role and function of ERAPs in peptide repertoire development. Specifically, our assay allows for monitoring trimming of free but also of MHC I-bound peptides which may reflect the physiological situation best.