Prevalence, phenotype and genotype of oral enterococci
Prevalence, phenotype and genotype of oral enterococci
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DOI：
10.1111/j.0902-0055.2004.00122.x
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发表时间：
2004-04-01
影响因子：
--
通讯作者：
Clewell, DB
中科院分区：
文献类型：
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作者：
Sedgley, CM;Lennan, SL;Clewell, DB
本研究调查了口腔肠球菌的流行情况、表型和基因型。在100名接受牙髓治疗的患者中有11%的口腔冲洗液样本中检测到肠球菌，在100名没有牙髓治疗史的牙科学生中有1%的口腔冲洗液样本中检测到肠球菌（P = 0.0027）。所有肠球菌分离株均鉴定为粪肠球菌。活菌计数范围为1 x 10 - 6 x 10（3）菌落形成单位/mL口腔冲洗液样品。口服E. faecalis菌株的溶血素和明胶酶的产生以及对E.粪培养滤液（n = 1）。6株菌株产生细菌素。所有菌株均对氨苄青霉素、苄青霉素、庆大霉素和万古霉素敏感。没有金属离子抗性的证据。一个分离物产生溶血素，明胶酶和细菌素，对几种抗生素有抗性，并对信息素cPD 1有反应。脉冲场凝胶电泳和质粒分析表明，口服E。粪肠球菌具有广泛的遗传多态性，在7株中检测到质粒。
This study investigated the prevalence, phenotype and genotype of oral enterococci. Enterococci were detected in oral rinse samples from 11% of 100 patients receiving endodontic treatment and 1% of 100 dental students with no history of endodontic treatment (P = 0.0027). All enterococcal isolates were identified as Enterococcus faecalis. Viable counts ranged from 1 x 10 to 6 x 10(3) colony forming units per mL of oral rinse sample. Potential virulence traits expressed by oral E. faecalis strains included production of hemolysin (n = 4) and gelatinase (n = 4), and response to pheromones in E. faecalis culture filtrate (n = 1). Six strains produced bacteriocin. All strains were susceptible to ampicillin, benzylpenicillin, gentamicin and vancomycin. There was no evidence of metal-ion resistance. One isolate produced hemolysin, gelatinase and bacteriocin, was resistant to several antibiotics, and responded to the pheromone cPD1. Pulsed-field gel electrophoresis and plasmid analysis showed that oral E. faecalis exhibited widespread genetic polymorphism, with plasmids detected in seven strains.