Substance P potentiates TGFbeta-1 production in lung epithelial cell lines.
Substance P potentiates TGFbeta-1 production in lung epithelial cell lines.
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P 物质增强肺上皮细胞系中 TGFbeta-1 的产生。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
T. Ghazanfari
中科院分区：
文献类型：
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作者：
R. Yaraee;T. Ghazanfari
转化生长因子- β (tgf - β)是一种重要的细胞因子，通过刺激细胞外基质沉积参与生长、分化、修复，并参与肺纤维化的发病机制。肺上皮细胞被认为是肺中tgf - β的来源。P物质（Substance P， SP）作为一种神经免疫调节剂在炎症气道中水平升高，虽然在肺纤维化的发病机制中有重要作用，但其对肺上皮细胞tgf - β生成的影响（及其调控潜力）尚不清楚。本研究检测了不同浓度SP存在下正常（BEAS-2B）和癌（A549）肺上皮细胞系培养的上清液中tgf - β -1的水平，并采用MTT法评估细胞活力。我们观察到SP（没有任何其他刺激剂）显著增加BEAS和A54细胞的tgf - β产生，这种作用被nk1受体拮抗剂（CP-96345）抑制。我们还观察到SP的存在对细胞的活力没有明显影响。由此可以推断，SP可以直接调节人支气管上皮细胞系tgf - β的释放，从而参与各种肺功能或病理状态。
Transforming growth factor-beta (TGF-beta) is one of the most important cytokines implicated in growth, differentiation, repair and also the pathogenesis of the lung fibrosis by its stimulatory effect on extracellular matrix deposition. Pulmonary epithelial cells are considered as a source of TGF-beta in lung. Substance P (SP), as a neuroimmunomodulator has elevated levels in inflamed airways and although it has significant role in the pathogenesis of the lung fibrosis, but its effect on transforming growth factor -beta (TGF-beta) production of the lung epithelial cells (and so its regulatory potential) remains unclear. In this study TGFbeta-1 levels in supernatants of the normal (BEAS-2B) and cancerous (A549) lung epithelial cell line cultures at the presence of various concentrations of SP were examined and MTT assay was performed to evaluate cells viability. We have observed that SP (without any other stimulator) significantly augments TGF-beta production of both BEAS and A54 cells and this effect is inhibited by NK1-receptor antagonist (CP-96345). We have also observed that the viability of cells did not significantly affect at the presence of SP. It can be concluded that SP can directly modulate the release of TGF-beta from human bronchial epithelial cell line and thereby participates in various lung functions or pathologic conditions.