Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures
Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures
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DOI：
10.1016/j.biomaterials.2004.06.047
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Seliktar, D
中科院分区：
文献类型：
--
作者：
Almany, L;Seliktar, D
组织工程支架由生物材料制成，提供生物功能信号并与细胞良好相互作用，或由合成聚合物制成，可对其结构特性进行精确控制。我们描述了一种生物合成混合支架，由纤维蛋白原主链组成，并与双功能聚乙二醇（PEG）侧链交联。变性的纤维蛋白原片段用 PEG-二丙烯酸酯进行聚乙二醇化，与光引发剂混合并暴露于紫外线下，在细胞悬浮液存在的情况下形成水凝胶材料。这种独特的水凝胶材料比其他支架材料具有明显的优势，因为它的机械性能具有高度可塑性，而生物功能则由聚合物网络的主链维持。 PEG-纤维蛋白原水凝胶的弹性模量取决于PEG成分的分子量并且与聚合物组合物的百分比成比例。纤维蛋白原骨架中的生物结构域为内皮细胞和平滑肌细胞粘附提供附着基序，并为生物降解提供蛋白水解敏感性。平滑肌细胞表现出蛋白水解渗透水凝胶材料并形成细胞互连网络的能力。我们致力于开发用于在 3D 环境中培养细胞的新型可生物降解支架，这有利于组织再生疗法。 (C) 2004 Elsevier Ltd. 保留所有权利。
Tissue engineering scaffolds are fabricated from either biological materials, which provide biofunctional signals and interact well with cells, or from synthetic polymers, which provide precise control over their structural properties. We describe a biosynthetic hybrid scaffold comprised of a fibrinogen backbone and crosslinked with difunctional polyethylene glycol (PEG) side chains. Denatured fibrinogen fragments are PEGylated with PEG-diacrylates, mixed with photoinitiator and exposed to UV light to form a hydrogel material in the presence of a cell suspension. This unique hydrogel material provides a distinct advantage over other;scaffold materials because its mechanical properties are highly malleable while the biological functionality is maintained by the backbone of the polymeric network. The elastic modulus of the PEG-fibrinogen hydrogel is dependent on the molecular weight of the PEG constituent and proportional to the percent polymeric composition. The biological domains in the fibrinogen backbone provide attachment motifs for endothelial cell and smooth muscle cell adhesion as well as proteolytic sensitivity for biodegradation. Smooth muscle cells demonstrate the ability to proteolytically penetrate through the hydrogel material and form interconnecting networks of cells. Our efforts to develop novel biodegradable scaffolds for cultivating cells in a 3D environment are beneficial for tissue regeneration therapies. (C) 2004 Elsevier Ltd. All rights reserved.