The expression of thromboxane A2 synthase and thromboxane A2 receptor gene in human uterus.
The expression of thromboxane A2 synthase and thromboxane A2 receptor gene in human uterus.
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DOI：
10.1095/biolreprod47.1.105
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发表时间：
1992-07
影响因子：
3.6
通讯作者：
M. L. Swanson;Z. Lei;P. Swanson;C. Rao;S. Narumiya;M. Hirata
中科院分区：
文献类型：
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作者：
M. L. Swanson;Z. Lei;P. Swanson;C. Rao;S. Narumiya;M. Hirata
应用免疫印迹、免疫细胞化学、北方印迹、原位杂交和放射自显影等方法，研究了人子宫血栓素（TX）A2合成酶和血栓素A2受体基因的表达。人子宫含有55 kDa的单一免疫反应性蛋白，其对应于人TXA 2合酶的分子大小。人子宫也含有一个2.8kb的TXA_2受体mRNA转录本和一个能与TXA_2拮抗剂125 I-PTA-OH结合的受体蛋白。免疫反应性TXA 2合成酶、TXA 2受体mRNA和蛋白存在于子宫内膜腺体、基质细胞、子宫肌层平滑肌和子宫血管中。不同子宫细胞中的血栓素A2合成酶和血栓素A2受体在不同生殖状态内以及不同生殖状态之间存在差异。在所有生殖状态下，除绝经后外，即使在细长和圆形子宫肌层平滑肌之间，结合位点数量也存在差异。总之，所提供的数据首次证明了不同的人子宫内膜和子宫肌层细胞以及子宫血管表达TXA 2合酶以及TXA 2受体基因。的表达，以及在各种生殖状态的变化，表明TXA 2可能是一个自分泌/旁分泌调节人子宫肌层收缩，子宫内膜分泌功能，和子宫内血流，并可能在妇女劳动的开始和/或进展中发挥作用。
The expression of thromboxane (TX) A2 synthase and thromboxane A2 receptor gene in human uterus was investigated by immunoblotting, immunocytochemistry, Northern blot, in situ hybridization, and autoradiographic analyses. Human uterus contains a single immunoreactive protein of 55 kDa that corresponds to the molecular size of human TXA2 synthase. Human uterus also contains a single 2.8-kb TXA2 receptor mRNA transcript and a receptor protein that can bind TXA2 antagonist, 125I-PTA-OH. The immunoreactive TXA2 synthase, TXA2 receptor mRNA, and protein are present in endometrial glands, stromal cells, myometrial smooth muscle, and uterine blood vessels. The TXA2 synthase and TXA2 receptors in different uterine cells varied within as well as between various reproductive states. There were differences in the binding site numbers even between elongated and circular myometrial smooth muscle in all reproductive states except postmenopause. In summary, the data presented demonstrate for the first time that different human endometrial and myometrial cells and uterine blood vessels express TXA2 synthase as well as TXA2 receptor gene. The expression, as well as changes during various reproductive states, suggests that TXA2 could be an autocrine/paracrine regulator of human myometrial contractions, endometrial secretory functions, and intrauterine blood flow and could play a role in the initiation and/or progression of labor in women.