Review and future prospects for DNA barcoding methods in forensic palynology
Review and future prospects for DNA barcoding methods in forensic palynology
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DOI：
10.1016/j.fsigen.2015.12.010
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发表时间：
2016-03-01
影响因子：
3.1
通讯作者：
Brosi, Berry J.
中科院分区：
文献类型：
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作者：
Bell, Karen L.;Burgess, Kevin S.;Brosi, Berry J.
在确定地理来源很重要的情况下，包括调查线索、失踪人员案件和情报应用，花粉可以成为关键的法医标记。然而，其使用此前一直受到孢粉学家需要高水平的专业化、识别速度慢以及分类学分辨率相对较差（通常达到植物科或属水平）的限制。相比之下，通过 DNA 条形码识别花粉有可能克服所有这三个限制，而且该方法尚未广泛实施似乎令人惊讶。尽管 DNA 条形码在花粉上的应用看似简单，但存在一些技术问题阻碍了进展。然而，花粉 DNA 条形码标准方法的最新发展，以及高通量测序技术的改进，已经克服了大部分技术问题。基于花粉 DNA 条形码的最新方法学进展，我们相信现在是开始将这些技术应用于法医孢粉学的时候了。在本文中，我们讨论了这些方法的潜力，并概述了未来研究的方向，以进一步改进该技术并提高其在更广泛情况下的适用性。 (C) 2015 Elsevier Ireland Ltd. 保留所有权利。
Pollen can be a critical forensic marker in cases where determining geographic origin is important, including investigative leads, missing persons cases, and intelligence applications. However, its use has previously been limited by the need for a high level of specialization by expert palynologists, slow speeds of identification, and relatively poor taxonomic resolution (typically to the plant family or genus level). By contrast, identification of pollen through DNA barcoding has the potential to overcome all three of these limitations, and it may seem surprising that the method has not been widely implemented. Despite what might seem a straightforward application of DNA barcoding to pollen, there are technical issues that have delayed progress. However, recent developments of standard methods for DNA barcoding of pollen, along with improvements in high-throughput sequencing technology, have overcome most of these technical issues. Based on these recent methodological developments in pollen DNA barcoding, we believe that now is the time to start applying these techniques in forensic palynology. In this article, we discuss the potential for these methods, and outline directions for future research to further improve on the technology and increase its applicability to a broader range of situations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.