Characterization of phosphorus forms in lake macrophytes and algae by solution 31P nuclear magnetic resonance spectroscopy
Characterization of phosphorus forms in lake macrophytes and algae by solution 31P nuclear magnetic resonance spectroscopy
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通过溶液 P-31 核磁共振波谱表征湖泊大型植物和藻类中的磷形态
DOI：
10.1007/s11356-015-5913-5
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发表时间：
2016-04-01
影响因子：
5.8
通讯作者：
Fan, Mingle
中科院分区：
文献类型：
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作者：
Feng, Weiying;Zhu, Yuanrong;Fan, Mingle
来自水生植物和藻类的残骸是磷 (P) 的重要回收来源，通过回收富营养化湖泊中的生物可利用磷，可导致藻类持续大量繁殖。然而，对水生植物和藻类中磷的形态及其对湖泊中磷内部负荷的贡献的了解有限。如果没有这些知识，就很难制定适当的策略来修复和/或恢复已变得富营养化的水生生态系统。因此，在这项工作中，从中国太湖采集的六种水生植物和藻类中提取了磷，并使用溶液 P-31 核磁共振 (NMR) 光谱进行了表征。当用0.5 M NaOH-25 mM EDTA萃取时，总P(TP)和有机P(P-o)的萃取回收率超过90%。藻类和水生植物中P-o浓度分别为5552 mg·kg(-1)和1005 mg·kg(-1),分别占TP的56.0%和47.2%。当P-o（包括缩合P）通过溶液P-31-NMR表征时，藻类中的P-o包括正磷酸单酯（79.8%）、焦磷酸（18.2%）和正磷酸二酯（2.0%），而水生植物中的P-o包括正磷酸单酯（90.3%）、焦磷酸（4.2%）和正磷酸二酯（5.5%）。 %）。此外，藻类碎片中的正磷酸单酯主要包括β-甘油磷酸（44.1%）、α-甘油磷酸（13.5%）和葡萄糖6-磷酸（13.5%）。水生植物中的正磷酸单酯主要包括β-甘油磷酸(27.9%)、α-甘油磷酸(24.6%)和5'单磷酸腺苷(8.2%)。这项研究的结果将有助于更好地了解淡水湖的养分循环、相关富营养化过程和污染控制。
Debris from aquatic macrophytes and algae are important recycling sources of phosphorus (P), which can result in continuing blooms of algae by recycling bioavailable P in the eutrophic lakes. However, knowledge of forms of P in aquatic macrophytes and algae and their contribution to internal loads of P in lakes is limited. Without such knowledge, it is difficult to develop appropriate strategies to remediate and or restore aquatic ecosystems that have become eutrophic. Therefore, in this work, P was extracted from six types of aquatic macrophytes and algae collected from Tai Lake of China and characterized by use of solution P-31-nuclear magnetic resonance (NMR) spectroscopy. When extracted by 0.5 M NaOH-25 mM EDTA, extraction recovery of total P(TP) and organic P(P-o) exceeded 90 %. Concentrations of P-o in algae and aquatic macrophytes were 5552 mg kg(-1) and 1005 mg kg(-1) and accounted for 56.0 and 47.2 % of TP, respectively. When P-o, including condensed P, was characterized by solution P-31-NMR P-o in algae included orthophosphate monoesters (79.8 %), pyrophosphate (18.2 %), and orthophosphate diester (2.0 %), and P-o in aquatic macrophytes included orthophosphate monoesters (90.3 %), pyrophosphate (4.2 %), and orthophosphate diester (5.5 %). Additionally, orthophosphate monoesters in algal debris mainly included beta-glycerophosphate (44.1 %), alpha-glycerophosphate (13.5 %), and glucose 6-phosphate (13.5 %). Orthophosphate monoesters in aquatic macrophytes mainly included beta-glycerophosphate (27.9 %), alpha-glycerophosphate (24.6 %), and adenosine 5' monophosphate (8.2 %). Results derived from this study will be useful in better understanding nutrient cycling, relevant eutrophication processes, and pollution control for freshwater lakes.