Folin-Ciocalteu Assay Inspired Polyoxometalate Nanoclusters as a Renal Clearable Agent for Non-Inflammatory Photothermal Cancer Therapy
Folin-Ciocalteu Assay Inspired Polyoxometalate Nanoclusters as a Renal Clearable Agent for Non-Inflammatory Photothermal Cancer Therapy
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Folin-Ciocalteu 试验启发多金属氧酸盐纳米簇作为肾脏可清除剂用于非炎症光热癌症治疗
DOI：
10.1021/acsnano.9b08894
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发表时间：
2020
期刊：
影响因子：
17.1
通讯作者：
Zhengbao Zha
中科院分区：
文献类型：
--
作者：
Junhong Zhou;Wancheng Zhao;Zhaohua Miao;Jingguo Wang;Yan Ma;Haitao Wu;Tiedong Sun;Haisheng Qian;Zhengbao Zha
与临床上的平移热消融技术类似，肿瘤光热治疗（PTT）过程中细胞坏死的发生会引起炎症反应，这对治疗结果不利。受Folin-Ciocalteu比色法的启发，在碱性水溶液中，通过与天然没食子酸的简单氧化还原反应，成功地制备了单分散、肾可清除的钨（W）基聚氧乙烯酸盐纳米簇（W-POM NC，平均直径约2.0 nm）。除冻干粉稳定性良好外，W-POM纳米粒在体内外对正常细胞/组织具有良好的生物相容性，在30 d内对Balb/c小鼠无明显毒性反应。更重要的是，W-POM NC不仅表现出相当大的近红外（NIR）光吸收（源于电子捕获的W5+的存在的着色效应），以实现有效的PTT，而且还表现出令人印象深刻的抗炎能力（通过将W5+氧化成W 6+状态来消除炎症相关的活性氧），以实现更好的治疗效果。因此，我们的研究开创了POM在非炎症PTT中的应用，具有预期的安全性和有效性。
Similar to translated thermal ablative techniques in clinic, the occurrence of cellular necrosis during tumor photothermal therapy (PTT) would induce inflammatory responses that are detrimental to therapeutic outcomes. Inspired by the well-known colorimetric Folin–Ciocalteu assay, monodispersed and renal-clearable tungsten (W)-based polyoxometalate nanoclusters (W-POM NCs, average diameter of around 2.0 nm) were successfully obtained here through a facile redox reaction with natural gallic acid in alkaline aqueous solution. Apart from excellent stability in the form of freeze-dried powder, the as-prepared W-POM NCs occupied considerable biocompatibility toward normal cells/tissues bothin vitroandin vivo, since no obvious toxicities were observed by treating female Balb/c mice with concentrated W-POM NCs during the 30 day post-treatment period. More importantly, W-POM NCs exhibited not only considerable near-infrared (NIR) light absorption (coloration effect originated from the existence of electron-trapped W5+) for efficient PTT but also impressive anti-inflammatory ability (eliminating inflammation-related reactive oxygen species by the oxidation of W5+into W6+state) to achieve better therapeutic outcomes. Thus, our study pioneers the application of POMs for non-inflammatory PTT with expected safety and efficiency.