Rio Mamore virus: Genetic characterization of a newly recognized hantavirus of the pygmy rice rat, Oligoryzomys microtis, from Bolivia
Rio Mamore virus: Genetic characterization of a newly recognized hantavirus of the pygmy rice rat, Oligoryzomys microtis, from Bolivia
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DOI：
10.4269/ajtmh.1997.57.368
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发表时间：
1997-09-01
影响因子：
3.3
通讯作者：
Hjelle, B
中科院分区：
文献类型：
--
作者：
Bharadwaj, M;Botten, J;Hjelle, B
人类汉坦病毒病遍布南美洲大部分地区。啮齿动物宿主和具体病原体很大程度上未知，但许多报道的病例发生在奥佐明啮齿动物（稻鼠）的居住范围内。我们已经在玻利维亚的侏儒稻 mt Oligoryzomys microtis 中鉴定出一种基因新颖的汉坦病毒（Rio Mamore 病毒 [RM]）。描述了小（S）基因组的完整序列和中（M）基因组的部分序列。该病毒与巴塔哥尼亚新发现的人类病原体安第斯病毒密切相关。为了促进南美洲汉坦病毒感染的改进诊断，我们在大肠杆菌中表达了 RM 的完整核衣壳蛋白，并对其进行亲和纯化，用于针对 RM 抗体的 ELISA 和蛋白质印迹测定。
Human hantavirus disease occurs throughout much of South America. The rodent hosts and the specific etiologic agent(s) are largely unknown, but many reported cases occurred within the habitation ranges of oryzomine rodents (rice rats). We have identified a genetically novel hantavirus (Rio Mamore virus [RM]) of the pygmy rice mt Oligoryzomys microtis in Bolivia. The complete sequence of the small (S) genome and the partial sequence of the medium (M) genome are described. This virus is closely related to the newly identified human pathogen Andes virus from Patagonia. To facilitate improved diagnosis of hantavirus infections in South America, we have expressed the complete nucleocapsid protein of RM in Escherichia coli and affinity-purified it for use in an ELISA and Western blot assays for antibodies to RM.