Sotta, Naoyuki, et al. "A low boron condition without high temperature stress induces internal browning in Raphanus sativus L.(Japanese radish)
Sotta, Naoyuki, et al. "A low boron condition without high temperature stress induces internal browning in Raphanus sativus L.(Japanese radish)
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索塔，直之，等人。
DOI：
10.1080/00380768.2022.2153346
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发表时间：
2023
影响因子：
2
通讯作者：
Toru Fujiwara
中科院分区：
文献类型：
--
作者：
Naoyuki Sotta;Satoshi Niikura ;Takehiro Kamiya;Toru Fujiwara
棕心，或内部褐变，是包括萝卜在内的作物中的一种生理障碍。它的特点是内部组织中的棕色色素沉着，这显著降低了产品的商业价值。田间研究表明，症状的出现与干旱、高温和缺硼等几种逆境有关。然而，现场实验中波动和不可重现的环境因素使对症状的直接原因的解释复杂化。为了研究症状的机制，最好在更可控的条件下进行实验，环境因素波动较小。在这里，我们建立了在实验室条件下观察棕心的培养方法，在实验室条件下，控制光、温度和营养。在我们的培养条件下，我们在没有高温和干旱胁迫的情况下，通过降低硼浓度成功地观察到了日本萝卜的内部褐变，表明缺硼本身就会导致内部褐变。此外，我们培养了三个品种，并观察到对内部褐变的敏感性差异，证明我们的培养方法适用于利用品种间变异的研究，如QTL定位。
Brown heart, or internal browning, is a physiological disorder in crops including radish. It is characterized by brown pigmentation in the internal tissue, which significantly reduces commercial value of the products. Field studies have revealed that the appearance of symptom is correlated with several stresses, including drought, high temperature, and boron deficiency. However, fluctuating and non-reproducible environmental factors in field experiments complicate the interpretation of the direct cause of the symptom. For studying the mechanism of the symptom, experiments under more controlled conditions with less fluctuating environmental factors are desirable. Here, we established culture method to observe brown heart in lab condition, where light, temperature, and nutrient is controlled. Under our culture conditions, we successfully observed internal browning in Japanese radish by lowering boron concentrations without heat and drought stresses, indicating that boron deficiency alone can cause internal browning. Furthermore, we cultured three cultivars and observed differences in susceptibility to internal browning, establishing that our culture method is applicable for studies that utilize inter-cultivar variations, such as QTL mapping.